Patent US3725075A successfully refreshed from Google Patents.
US3725075A
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Method of recovering proteins from microbial cells
Assignee: Hitachi Ltd
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Filing: 1971-10-28
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Granted: 1973-04-03
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Expires: 1991-10-28
Abstract
Abstract A METHOD OF RECOVERING PROTEINS FROM MICROBIAL CELLS WHICH COMPRISES PREPARING AN EXTRACT CONTAINING PROTEINS AND SEPARATING THE PROTEINS BY ISOELECTRIC POINT PRECIPITAUON FROM THE EXTRACT, SAID ISOELECTRIC POINT PRECIPITATION BEING PERFORMED IN THE PRESENCE OF A WATER SOLUBLE SALT SELECTED FROM THE GROUP CONSISTING OF ALKALI METAL SALTS AND AMMONIUM SALTS IN SUCH AN AMOUNT THAT THE IONIC STRENGTH OF THE SALT RANGES BETWEEN 0.002 AND 0.2 WHEREBY PRECIPITATES OF PROTEINS AGGREGATE TO GROW IN SIZE AND THE PRECIPITATES CAN BE SEPARATED EASILY TO INCREASE A YIELD OF PROTEIN RECOVERY.
Inventors
- 👤 T Muroi
- 👤 Y Oguri
- 👤 M Ishida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:42
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/005; A; A23; A23J; A23J1/00; A23J1/008; A23J1/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/911; Y10S435/921; Y10S435/923; Y10S435/946; Y10S435/947; Y10S530/00; Y10S530/82; Y10S530/822; Y10S530/823; Y10S530/824; Y10S530/825
Claims Analysis
Total Claims: 0
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References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-04-02 | 1976-10-02 | 1977-04-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-04-02 | 1980-10-02 | 1981-04-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-04-02 | 1984-10-02 | 1985-04-03 | $7700.00 | Window Closed / Lapsed |