US3717550A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Liquid compositions of bacterial protease and/or amylase and preparation thereof
Assignee: PMP ACQUISITION Corp A DE CORP
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Filing: 1970-09-25
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Granted: 1973-02-20
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Expires: 1990-09-25
Abstract
Abstract Liquid concentrates of bacterial protease and/or amylase of enhanced stability are prepared. Bacterial protease and/or amylase liquid compositions free of turbidity and a process of producing them are provided. These compositions have advantages over powdered enzymes in avoiding atmospheric contamination and are especially useful in detergents and in textile treatment.
Inventors
- 👤 J Ziffer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 19:12
CPC Classifications
D; D06; D06L; D06L1/00; D06L1/12; D06L1/14; C; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38663
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
131 words
A liquid composition consisting essentially of: Component (A), fermentation soluble solids consisting essentially of an enzyme from the group consisting of bacterial protease and bacterial amylase and mixtures thereof, and Component (B), a liquid from the group consisting of water, a normally liquid, water miscible polyhydric alcohol, and both water aNd a normally liquid, water miscible polyhydric alcohol, in which said enzyme is soluble to the extent of at least 32% fermentation soluble solids by weight at 4.4*C., said composition containing a concentration of said components sufficient to maintain an enzyme activity of at least 50% when said composition is stored in a container under atmospheric pressure for six days at 42*C., Component (A) constituting at least 32% by weight of the solids content of said composition, on a dry basis.
Claim 2
Dependent Claim
131 words
A liquid composition consisting essentially of: Component (A), fermentation soluble solids consisting essentially of an enzyme from the group consisting of bacterial protease and bacterial amylase and mixtures thereof, and Component (B), a liquid from the group consisting of water, a normally liquid, water miscible polyhydric alcohol, and both water aNd a normally liquid, water miscible polyhydric alcohol, in which said enzyme is soluble to the extent of at least 32% fermentation soluble solids by weight at 4.4*C., said composition containing a concentration of said components sufficient to maintain an enzyme activity of at least 50% when said composition is stored in a container under atmospheric pressure for six days at 42*C., Component (A) constituting at least 32% by weight of the solids content of said composition, on a dry basis.
Claim 3
Dependent Claim
22 words
A composition as claimed in claim 1 in which the protease activity is at least 5,000 PV units per gram at 4.4*C.
Claim 4
Dependent Claim
22 words
A composition as claimed in claim 1 in which the protease activity is 5,000 to 2,000,000 PV units per gram at 4.4*C.
Claim 5
Dependent Claim
22 words
A composition as claimed in claim 1 in which the protease activity is 30,000 to 600,000 PV units per gram at 4.4*C.
Claim 6
Dependent Claim
22 words
A composition as claimed in claim 1 in which the amylase activity is at least 10,000 DV units per gram at 4.4*C.
Claim 7
Dependent Claim
22 words
A composition as claimed in claim 1 in which the amylase activity is 10,000 to 1,000,000 DV units per gram at 4.4*C.
Claim 8
Dependent Claim
22 words
A composition as claimed in claim 1 in which the amylase activity is 30,000 to 400,000 DV units per gram at 4.4*C.
Claim 9
Dependent Claim
29 words
A composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32% by weight to saturation.
Claim 10
Dependent Claim
30 words
A composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32 to 43.5 percent by weight.
Claim 11
Dependent Claim
37 words
A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial protease, and component (B) consists essentially of glycerol.
Claim 12
Dependent Claim
37 words
A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial amylase, and component (B) consists essentially of glycerol.
Claim 13
Dependent Claim
41 words
A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of both bacterial protease and bacterial amylase, and component (B) consists essentially of glycerol.
Claim 14
Dependent Claim
39 words
A composition as claimed in claim 1 in which said enzyme is present in a concentration of at least 32 percent by weight, on a dry basis, and said liquid is from the group consisting of glycerol and water.
Claim 15
Dependent Claim
62 words
A process of clarifying an aqueous solution of an enzyme from the group consisting of bacterial protease, bacterial amylase and mixtures thereof which comprises adding to a turbid aqueous solution in which the concentration of said enzyme is at least 32 percent by weight, on a dry basis, a sufficient amount of a liquid, water miscible polyhydric alcohol to reduce the turbidity.
Claim 16
Dependent Claim
62 words
A process of clarifying an aqueous solution of an enzyme from the group consisting of bacterial protease, bacterial amylase and mixtures thereof which comprises adding to a turbid aqueous solution in which the concentration of said enzyme is at least 32 percent by weight, on a dry basis, a sufficient amount of a liquid, water miscible polyhydric alcohol to reduce the turbidity.
Claim 17
Dependent Claim
14 words
A process as claimed in claim 14 in which said polyhydric alcohol is glycerol.
Claim 18
Dependent Claim
14 words
A process as claimed in claim 14 in which said enzyme is bacterial protease.
Claim 19
Dependent Claim
36 words
A process as claimed in claim 14 in which said aqueous solution containing said polyhydric alcohol is concentrated under conditions facilitating the elimination of at least a part of the water without removing said polyhydric alcohol.
Claim 20
Dependent Claim
18 words
A process as claimed in claim 17 in which said concentration is carried out under subatmospheric pressure conditions.
Claim 21
Dependent Claim
14 words
A process as claimed in claim 14 in which said enzyme is bacterial amylase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-02-20 | 1976-08-21 | 1977-02-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-02-20 | 1980-08-21 | 1981-02-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-02-20 | 1984-08-21 | 1985-02-20 | $7700.00 | Window Closed / Lapsed |