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US3715276A
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Enzymatic method for manufacture of fructose from glucose
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1970-10-23
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Granted: 1973-02-06
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Expires: 1990-10-23
Abstract
Abstract In an enzymatic reaction for isomerizing glucose into fructose with a glucose isomerase, a method is provided for converting glucose into fructose which is characterized by using calcium carbonate, magnesium carbonate an anion-exchange resin or an amphoteric-ion exchange resin as a pH regulator.
Inventors
- 👤 Y Takasaki
- 👤 A Kamibayashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 20:29
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/24
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
91 words
In the method of enzymatically converting glucose into fructose in a glucose-containing reaction solution containing glucose isomerase, the improvement comprising admixing with said reaction solution at least one reagent selected from the group consisting of magnesium carbonate, calcium carbonate, an anion-exchange resin and an amphoteric-ion exchange resin, and converting glucose to fructose in admixture with said reagent during the entire course of said conversion without regulating the pH of the reaction solution by addition of alkali whereby said reagent regulates the pH and suppresses the non-enzymatic conversion of sugar to acid.
Claim 2
Dependent Claim
91 words
In the method of enzymatically converting glucose into fructose in a glucose-containing reaction solution containing glucose isomerase, the improvement comprising admixing with said reaction solution at least one reagent selected from the group consisting of magnesium carbonate, calcium carbonate, an anion-exchange resin and an amphoteric-ion exchange resin, and converting glucose to fructose in admixture with said reagent during the entire course of said conversion without regulating the pH of the reaction solution by addition of alkali whereby said reagent regulates the pH and suppresses the non-enzymatic conversion of sugar to acid.
Claim 3
Dependent Claim
21 words
A method as set forth in claim 1 wherein said reaction solution has a pH of between about 6 and 9.
Claim 4
Dependent Claim
44 words
A method as set forth in claim 1 wherein said glucose-containing isomerase and said reaGent are packed in a column and a glucose-containing solution is passed through said column to form said reaction solution and said glucose is converted into fructose in said column.
Claim 5
Dependent Claim
21 words
A method as set forth in claim 3 wherein said reaction solution has a pH of between about 6 and 9.
Claim 6
Dependent Claim
14 words
A method as set forth in claim 4 wherein said reagent is magnesium carbonate.
Claim 7
Dependent Claim
14 words
A method as set forth in claim 4 wherein said reagent is calcium carbonate.
Claim 8
Dependent Claim
15 words
A method as set forth in claim 4 wherein said reagent is an anion-exchange resin.
References Cited (Backward Citations)
US3050444A
Cited
US3616221A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-02-06 | 1976-08-07 | 1977-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-02-06 | 1980-08-07 | 1981-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-02-06 | 1984-08-07 | 1985-02-06 | $7700.00 | Window Closed / Lapsed |