System for the solid-phase peptide synthesis

Abstract

Abstract APPARATUS FOR THE SOLID-PHASE PEPTIDE SYNTHESIS WHICH COMPRISES A REACTION VESSEL CONSISTING OF A FLASK HAVING A SIDE ARM DEFINING AN INLET THROUGH WHICH A POLYMER CAN BE INSERTED INTO THE FLASK. MEANS ARE PROVIDED TO SEPARATELY INSERT A SOLVENT AND A REAGENT INSIDE THE FLASK WHILE THEY ARE DISTRIBUTED UNIFORMLY ALONG THE INNER WALLS OF THE FLASK. AT THE BOTTOM OF FLASK, THERE ARE MEANS TO REMOVE THE SOLVENT AND WASTE PRODUCTS FROM THE FLASK. PREFERABLY BY SUCTION. THE FLASK HAS AN OPENING TO PERMIT AN ENTRY OF AIR INSIDE THE FLASK IN ORDER TO CONTROL THE PRESSURE INSIDE THE FLASK AND TO FACILITATE REMOVAL OF SOLVENT AND WASTE PRODUCTS. THE APPARATUS ALSO COMPRISES MEANS ASSOCIATED WITH THE REACTION VESSEL TO SHAKE THE LATTER AND TO CAUSE SYNTHESIS OF THE PEPTIDE TO TAKE PLACE. THE SOLVENT AND REAGENT ARE CONTAINED IN RESERVOIRS WHICH ARE CONNECTED TO THE REACTION VESSEL AT A POINT WHERE THE LATTER SEPARATELY INTRODUCES SOLVENT AND REAGENT THEREIN, FINALLY, MEANS ARE PROVIDED TO CLEAVE THE PEPTIDE WHEN THE SYNTHESIS IS TERMINATED.

Inventors

• 👤 W Park
• 👤 D Regoli

Bibliographic Details