Patent US5039191A successfully refreshed from Google Patents.
Patent US3697645A successfully refreshed from Google Patents.
US3697645A
Expired / Lapsed
US
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Purification of antibodies
Assignee: United States Department of the Army
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Filing: 1971-04-28
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Granted: 1972-10-10
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Expires: 1991-04-28
Abstract
Abstract Recovering the entire spectrum of all heterogeneous antibodies against a specific antigen or ligand by a process of adding an antigen to an immune serum forming a precipitate which is dissociated by a combination of addition of excess hapten and a non-specific dissociating compound and separating the dissociated heterogeneous antibodies from the antigen, hapten and dissociating compounds added.
Inventors
- 👤 Eugene P Meier
- 👤 David E Lenz
- 👤 Ludwig A Sternberger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 09:40
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/44; Y; Y10; Y10S; Y10S530/00; Y10S530/807
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
25 words
The method according to claim 1, wherein the acid is HCl and the liquid medium containing the dissociated precipitate is at pH 1 to 4.
Claim 2
Dependent Claim
28 words
The method according to claim 1, wherein the alkali is an alkaline metal hydroxide and the liquid medium containing the dissociated precipitate is at pH 11 to 13.
Claim 3
Dependent Claim
24 words
The method according to claim 1, wherein the salt is urea and the liquid medium is 5 molar to 7 molar in said salt.
Claim 4
Dependent Claim
115 words
The method according to claim 1, wherein the antigen added to the immune serum is selected from the group consisting of diazotized p-aminophenyl diethylphosphate coupled into hemocyanin, diazotized 0-(p-aminophenyl)-0''-pinacolyl methylphosphonate coupled into hemocyanin, diazotized p-aminobenzoic acid coupled into hemocyanin, diazotized glucosamine coupled into hemocyanin, diazotized glycylglycine coupled into hemocyanin, diazotized p-amino-m-nitrophenol coupled into hemocyanin, and diazotized 0,0''-diethyl-0''''-p-aminophenylthiophosphate coupled into hemacyanin, the hapten is selected from the group consisting of p-aminophenyl diethylphosphate . HCl, 0-(p-aminophenyl)-0-pinacolyl methylphosphonate . HCl, p-aminobenzoic acid . HCl, glucosamine . HCl, glycylglycine . HCl, p-amino-m-nitrophenyl . HCl, and 0,0''-diethyl-0''''p-aminophenylthiophosphate . HCl, an the moieties are selected from the group consisting of diethyl-p-nitrophenyl phosphate, o-pinacolyl methylfluorophosphate, benzoic acid, glucosamine, glycylglycine, dinitrophenol and 0,0''-diethyl-0''''-p-nitrophenylthiophosphate.
Claim 5
Dependent Claim
17 words
The method according to claim 5, wherein the step of separating the soluble complex is gel filtration.
Claim 6
Dependent Claim
16 words
The method according to claim 5, wherein the step of separating the soluble complex is dialysis.
Claim 7
Dependent Claim
31 words
The method according to claim 7, wherein the antigen is diazotized p-aminophenyl diethylphosphate coupled into hemocyanin, the hapten is p-aminophenyl diethylphosphate . HCl and the moiety of organophosphate is diethyl-p-nitrophenyl phosphate.
Claim 8
Dependent Claim
31 words
The method according to claim 7, wherein the antigen is diazotized 0-(p-aminophenyl)-0''-pinacolyl methylphosphate coupled into hemocyanin, the hapten is 0-(p-aminophenyl)-0''-pinacolyl methylphosphate . HCl and the moiety of organophosphate is 0-pinacolyl methylfluorophosphate.
Claim 9
Dependent Claim
28 words
The method according to claim 7, wherein the antigen is diazotized 0,0''-diethyl-0''''-p-aminophenylthiophosphate coupled into hemocyanin, the hapten is 0,0''-diethyl-0''''-p-aminophenylthiophosphate . HCl, and the moiety of organophosphate is 0,0''-diethyl-0''''-p-nitrophenylthiophosphate.
Claim 10
Dependent Claim
140 words
A total heterogeneous antibody population against a specific poisoner produced by adding an antigen to an immune serum comprising antibody to said antigen and forming an immune precipitate in an aqueous medium, dissociating said precipitate with excess hapten, adding to the aqueous medium comprising the dissociated precipitate a compound of acid, alkali or saLt preventing reassociation of the dissociated precipitate and thereafter neutralizing the aqueous medium forming an insoluble antigen and soluble complex of antibody with hapten, centrifuging the neutralized aqueous medium and removing the insoluble antigen with the medium consisting essentially of the soluble complex, separating the antibody from the hapten by dialyzing the soluble complex in a semipermeable membrane against a physiologic buffer to the antibody and removing the aqueous medium consisting essentially of the heterogeneous antibody population from the interior of the membrane so obtained from dialysis.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-10-10 | 1976-04-10 | 1976-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-10-10 | 1980-04-10 | 1980-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-10-10 | 1984-04-10 | 1984-10-10 | $7700.00 | Window Closed / Lapsed |