Patent US3697638A successfully refreshed from Google Patents.
US3697638A
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Antigens
Assignee: F Hoffmann La Roche AG
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Filing: 1971-04-12
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Granted: 1972-10-10
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Expires: 1991-04-12
Abstract
Abstract Antigens associated with carcinomas and adenocarcinomas as well as methods for isolating, identifying and detecting them are disclosed.
Inventors
- 👤 Hans John Hansen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 17:38
CPC Classifications
B; B01; B01J; B01J47/00; B01J47/02; B01J47/04; A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/10; A61K51/1045; A61K51/1048; B01J39/00; B01J39/26; B01J47/014; C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; C07K16/3007; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57565; A61K2123/00; A61K39/00; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/813; Y10S436/825; Y10S530/00; Y10S530/806; Y10S530/827; Y10S530/828
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
15 words
The method of claim 1 wherein in step (a) the buffer is a borate buffer.
Claim 2
Dependent Claim
14 words
The method of claim 1 wherein in step (a) the buffer is ammonium acetate.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein in step (a) a perchloric acid extract of blood plasma is used.
Claim 4
Dependent Claim
24 words
The method of claim 1 wherein in step (c) the amount of CEA material, component A or component B added is about 50 nanograms.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein the in step (e) the precipitating agent is zirconyl phosphate gel.
Claim 6
Dependent Claim
16 words
The method of claim 1 wherein in step (c) radioactive tagged CEA component A is added.
Claim 7
Dependent Claim
16 words
The method of claim 1 wherein in step (c) radioactive tagged CEA component B is added.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein in step (c) radioactive tagged CEA material is added.
Claim 9
Independent Claim
153 words
A method for detecting the presence of carcinoembryonic antigen material, CEA component A and/or CEA component B in blood which comprises a. adding a measured amount of tagged CEA material, component A or component B to a perchloric acid extract of blood serum or blood plasma prior to dialysis; b. dialyzing the mixture against a polyethylene glycol with an average molecular weight of about 15,000 to 20,000 and a softening point of about 60* C.; c. adjusting the pH of the retentate to about pH 6-7; d. adding a measured amount of antibody to the adjusted mixture; e. dialyzing against polyethylene glycol of molecular weight of about 15,000 to 20,000 and having a softening point at 60* C.; f. dissolving the resulting retentate in borate buffer of pH 6.25; g. adding a precipitating agent to the solution thereby precipitating all the CEA-anti CEA complexes; and h. measuring the radioactive content of the precipitate.
Claim 10
Dependent Claim
23 words
The method of claim 10 wherein in step (c) the pH of the mixture is adjusted by a borate buffer of pH 6.25.
Claim 11
Dependent Claim
16 words
The method of claim 10 wherein in step (f) the precipitating agent is zirconyl phosphate gel.
Claim 12
Dependent Claim
15 words
The method of claim 10 wherein in step (a) I125 tagged CEA material is used.
Claim 13
Dependent Claim
15 words
The method of claim 10 wherein in step (a)I125 tagged CEA component A is used.
Claim 14
Dependent Claim
16 words
The method of claim 10 wherein in step (a) I125 tagged CEA component B is used.
Claim 15
Dependent Claim
13 words
The method of claim 10 wherein in step (a) blood plasma is used.
Claim 16
Independent Claim
97 words
A method for detecting the presence of carcinoembryonic antigen material, component A and/or component B in blood which comprises a. diluting blood serum or blood plasma with at least 100 volumes of water; b. adding a measured amount of CEA antiserum to the dilution; c. incubating the mixture; d. adding a measured amount of radioactive tagged carcinoembryonic antigen material, component A or component B to the incubated mixture; e. incubating the mixture; f. adding a precipitating agent to the incubating mixture thereby precipitating all the CEA-anti CEA complexes; and g. measuring the radioactive content of the precipitate.
Claim 17
Dependent Claim
16 words
The method of claim 17 wherein in step (f) the precipitating agent is zirconyl phosphate gel.
Claim 18
Dependent Claim
182 words
Radioiodinated carcinoemryonic antigen component A characterized by having a molecular weight of between 120,000 and 240,000, being elutable from a mixed bed ion exchange column comprising an equal mixture by weight of either preswollen or dry microgranular carboxymethyl cellulose having rod shaped particles with a particle size distribution with a range of about 20 Mu to about 60 Mu , a capacity of 1.0 + or - 0.1 meq./gm. and a water regain of 2.3-2.7 gm./gm. dry exchanger, and microgranular diethylaminoethyl cellulose, in free base form, having rod shaped particles, a particle size distribution with a range of about 20 Mu to about 60 Mu , a capacity of 1.0 + or - 0.1 meq./gm. and a water regain of 2.3-2.8 gm./gm. dry exchanger with ammonium acetate at pH 4 in 0.05 M sodium chloride, forming a single line precipitate with its specific antibody in unabsorbed antiserum in gel diffusion tests, being soluble in perchloric acid, having a spectrophotometer absorption peak wave length of 280 m Mu and having a specific activity of 5-10 m Mu Ci/ng. of carcinoembryonic antigen component A.
Claim 19
Dependent Claim
181 words
Radioiodinated carcinoembryonic antigen component B characterized by having a molecular weight of between 120,000 and 240,000 being elutable from a mixed bed ion exchange column comprising an equal mixture by weight of either preswollen or dry microgranular carboxymethyl cellulose having rod shaped particles with a particle size distribution with a range of about 20 Mu to about 60 Mu , a capacity of 1.0 + or - 0.1 meq./gm. and a water regain of 2.3-2.7 gm./gm. dry exchanger, and microgranular diethylaminoethyl cellulose, in free base form, having rod shaped particles, a particle size distribution with a range of about 20 Mu to about 60 Mu , a capacity of 1.0 + or - 0.1 meq./gm. and a water regain of 2.3-2.8 gm./gm. dry exchanger With ammonium acetate at pH 4 in 0.1 M NaCl, forming a single line precipitate with its specific antibody in unabsorbed antiserum in gel diffusion tests, being soluble in perchloric acid, having a spectrophotometer absorption peak wave length of 280 m Mu and having a specific activity of 5-10 m Mu Ci/ng. of carcinoembryonic antigen component B.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-10-10 | 1976-04-10 | 1976-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-10-10 | 1980-04-10 | 1980-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-10-10 | 1984-04-10 | 1984-10-10 | $7700.00 | Window Closed / Lapsed |