US3687927A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Orgotein isolation process employing single ion exchange resin having both acidic and basic groups
Assignee: GT Biopharma Inc
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Filing: 1971-06-07
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Granted: 1972-08-29
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Expires: 1991-06-07
Abstract
Abstract An improved process for reducing the divalent metal ion content to less than 10 7 M concentration of a solution of orgotein containing greater than 10 7 M concentration of a divalent metal having an ionic radius from 0.65 to 0.79 A, which comprises reducing the ion content to less than 10 7 M by passing the buffer solution having an ion content greater than 10 7 M through an ion exchange resin bed consisting of a single ion exchange resin having both acidic and basic groups which are in sufficiently close proximity so that the resin is partially neutralized internally yet sufficiently separated spatially so that the resin has absorptive attraction for both anions and cations.
Inventors
- 👤 Wolfgang Huber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 01:42
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0089; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/843; Y10S530/846
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
23 words
A process according to claim 1 wherein the resin is a styrene-divinyl, benzene gel-type resin containing -SO3H and -N(CH3)2(CH2OH)OH groups as ion-adsorptive groups.
Claim 2
Dependent Claim
17 words
A process according to claim 1 wherein the resin has a particle size of over 325 mesh.
Claim 3
Dependent Claim
28 words
A process according to claim 2 wherein the resin has a particle size of over 325 mesh and the ratio of resin to protein is at least 10:1.
Claim 4
Dependent Claim
32 words
A process according to claim 1 wherein the starting buffer solution contains a mixture of Mg , Cu and Zn ions as substantially the sole divalent metal ions in the buffer solution.
Claim 5
Dependent Claim
32 words
A process according to claim 4 wherein the starting buffer solution contains a mixture of Mg , Cu and Zn ions as substantially the sole divalent metal ions in the buffer solution.
Claim 6
Dependent Claim
36 words
A process according to claim 5 wherein the Mg , Cu and Zn ions are present in the starting buffer solution at concentrations of about 10 3 M, 10 4 M and 10 5 M, respectively.
Claim 7
Dependent Claim
42 words
A process according to claim 1 wherein the starting buffer solution is an eluate fraction obtained by filtration of a buffer solution containing a mixture of the orgotein protein and undesired proteins through a porous gel which acts as a molecular sieve.
Claim 8
Dependent Claim
36 words
A process according to claim 8 wherein the buffer solution has a pH of about 7.8 and contains about 10 3 M Mg , about 10 4 M Cu and about 10 5 M Zn ions.
Claim 9
Dependent Claim
46 words
A process according to claim 8 wherein the orgotein protein of the starting mixture of the orgotein protein and undesired proteins is a metal chelate wherein the metals thereof consist essentially of one or more divalent metals having an ionic radius from 0.65 to 0.79 A.
Claim 10
Dependent Claim
26 words
A process according to claim 10 wherein the metals of the divalent metal consist essentially of one or more of Mg , Cu and Zn .
Claim 11
Dependent Claim
74 words
A process according to claim 10 wherein the metal chelate of the starting mixture is obtained by transchelating the orgotein protein as a mixture with undesired proteins wherein the predominate metal of the metal chelate is Mn by dissolving the mixture of proteins in a buffer solution containing dissolved therein at a concentration greater than 10 7 M of a salt of a divalent metal having an ionic radius from 0.65 to 0.79 A.
Claim 12
Dependent Claim
31 words
A process according to claim 12 wherein the buffer solution contains dissolved therein at a concentration greater than 10 7 M of a mixture of Mg , Cu and Zn ions.
Claim 13
Dependent Claim
32 words
A process according to claim 13 wherein the Mg , Cu and Zn ions are present in the buffer solution at concentrations of about 10 3, 10 4 and 10 5, respectively.
References Cited (Backward Citations)
US2461505A
Cited
US2669559A
Cited
US2663668A
Cited
US3073747A
Cited
NL6614177A
Cited
GB1160151A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-08-29 | 1976-02-28 | 1976-08-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-08-29 | 1980-02-28 | 1980-08-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-08-29 | 1984-02-28 | 1984-08-29 | $7700.00 | Window Closed / Lapsed |