Ion exchange process for separating proteins

Claim 1

IN A PROCESS OF SEPARATING A PROTEIN FROM OTHER PROTEINS IN AN AQUEOUS SOLUTION CONTAINING A PLURALITY OF PROTEINS A SOLUTES AND ALSO HAVING A SALT CONTENT PRESENT AS CATIONS AND ANIONS IN A CONCENTRATION RENDERING SAID FIRST-MENTIONED PROTEIN SOLUBLE IN SAID SOLUTION AND IN THE ABSENCE OF WHICH CATIONS AND ANIONS SAID PROTEIN IS INSOLUBLE IN SAID SOLUTION, ANOTHER PROTEINS SOLUTE OF SAID SOLUTION BEING SOLUBLE THEREIN IN THE ABSENCE OF SAID CATIONS AND ANIONS IN SAID CONCENTRATION, THE STEPS OF PROVIDING A CATION-EXCHANGE RESIN AND AN ANION-EXCHANGE RESIN EACH CONDITIONED TO BE IN EQUILIBRIUM WITH THE ANION CONCENTRATION AT WHICH SAID SOLUTION-CONTAINED CATIONS AND ANIONS ARE RESPECTIVELY REMOVED FROM SAID SOLUTION BEING TREATED SO THAT SAID RESINS WILL ABSORB FROM SOLUTION SAID CORRESPONDING IONS, PASSING SAID AQUEOUS SOLUTION OF SAID PROTEINS AND SALT SOLUTE ALTERNATELY IN CONTACT WITH SAID CATIONS-EXCHANGE RESIN AND WITH SAID ANION-EXCHANGE RESIN WHILE HOLDING THE PH WITH EACH RESIN BETWEEN LIMITS EFFECTIVE TO WORK THE DESIRED ION REMOVAL FOR THE CORRESPONDING EXCHANGE AND WITHIN THE RANGE PH 1 TO PH 6 AND THUS REMOVING FROM SAID SOLUTIONS SAID SOLUBILIZING CATIONS BY ION EXCHANGE WITH SAID CATION-EXCHANGE RESIN TO REDUCE SAID SOLUBILIZING CATIONS BY ION EXCHANGE WITH SAID CATIONS-EXCHANGE RESIN AND REMOVING FROM SAID SOLUTION SAID SOLUBILIZING ANIONS BY ION EXCHANGE WITH SAID ANION-EXCHANGE RESIN TO REDUCE SAID SOLUBILIZING ANIONS, AND CONTINUING SAID ALTERNATE EXCHANGES TO REDUCE THE IONIC STRENGTH OF THE SOLUTION THEREBY PRECIPITATING SAID FIRST-MENTIONED PROTEIN FROM SAID SOLUTION OF SAID PROTEINS, AND SEPARATING SAID PRECIPITATE FROM SAID SOLUTION.