Patent US1584126A successfully refreshed from Google Patents.
Patent US3669841A successfully refreshed from Google Patents.
US3669841A
Expired / Lapsed
US
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Attachment of enzymes to siliceous materials
Assignee: Monsanto Co
•
Filing: 1970-02-11
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Granted: 1972-06-13
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Expires: 1990-02-11
Abstract
Abstract Enzymes are attached to siliceous materials by silation of the siliceous material to introduce functional groups which are then linked to an enzyme by means of crosslinking agents. The products are enzymatically active heavy-duty composites in which the enzyme is covalently attached via the crosslinking agent and an organosilane to the siliceous material.
Inventors
- 👤 Robert E Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 21:55
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12N11/02; C12N11/06; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
10 words
The composite of claim 1, wherein the organosilane is omega-aminoalkyltrialkoxysilane.
Claim 2
Dependent Claim
11 words
The composite of claim 2, wherein the omega-aminoalkyltrialkoxysilane is gamma-aminopropyl trimethoxysilane.
Claim 3
Dependent Claim
11 words
The composite of claim 1, wherein the crosslinking agent is formaldehyde.
Claim 4
Dependent Claim
11 words
The composite of claim 1, wherein the enzyme is a protease.
Claim 5
Dependent Claim
11 words
The composite of claim 1, wherein the siliceous material is silica.
Claim 6
Dependent Claim
18 words
The composite of claim 1, wherein the enzyme comprises a mixture of neutral protease, alkaline protease, and amylase.
Claim 7
Dependent Claim
13 words
The composite of claim 1, wherein the crosslinking agent is ethylene glycol bispropiolate.
Claim 8
Dependent Claim
12 words
The product of claim 1, wherein the siliceous material is calcium silicate.
Claim 9
Dependent Claim
11 words
The product of claim 1, wherein the crosslinking agent is glutaraldehyde.
Claim 10
Dependent Claim
23 words
The product of claim 1, wherein the enzyme comprises a neutral protease, alkaline protease, and amylase mixture produced by a Bacillus subtilis species.
Claim 11
Dependent Claim
104 words
Process for the production of an insoluble, enzymatically-active composite comprising the steps of reacting a siliceous material with a hydrolyzable group of an organosilane bearing a functional group and attaching an enzyme having a reactive group not essential for enzymatic activity to the reacted organosilane by reacting said reactive group with a crosslinking agent and reacting said crosslinking agent with the functional group of said organosilane, said crosslinking agent being a member of the group consisting of an aldehyde selected from the group consisting of formaldehyde, unsaturated monoaldehyde containing up to 4 carbon atoms, inclusive, and a dialdehyde; a bispropiolate; and a disulfonyl halide.
Claim 12
Dependent Claim
10 words
Process of claim 12, wherein the siliceous material is silica.
Claim 13
Dependent Claim
9 words
Process of claim 12, wherein the organosilane is omega-aminoalkyltrialkoxysilane.
Claim 14
Dependent Claim
9 words
Process of claim 12, wherein the organosilane is gamma-aminopropyltrimethoxysilane.
Claim 15
Dependent Claim
10 words
Process of claim 12, wherein the crosslinking agent is formaldehyde.
Claim 16
Dependent Claim
12 words
Process of claim 12, wherein the crosslinking agent is ethylene glycol bispropiolate.
Claim 17
Dependent Claim
10 words
Process of claim 12, wherein the crosslinking agent is glutaraldehyde.
Claim 18
Dependent Claim
10 words
Process of claim 12, wherein the enzyme comprises a protease.
Claim 19
Dependent Claim
14 words
Process of claim 12, wherein the enzyme comprises neutral protease, alkaline protease, and amylase.
Claim 20
Dependent Claim
22 words
Process of claim 12, wherein the enzyme comprises a neutral protease, alkaline protease and amylase mixture produced by a Bacillus subtilis species.
References Cited (Backward Citations)
US3519538A
Cited
US3502545A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-06-13 | 1975-12-13 | 1976-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-06-13 | 1979-12-13 | 1980-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-06-13 | 1983-12-13 | 1984-06-13 | $7700.00 | Window Closed / Lapsed |