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US3645853A
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Diagnostic composition and method for the detection of nitrate reduction
Assignee: Warner Lambert Co LLC
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Filing: 1969-06-24
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Granted: 1972-02-29
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Expires: 1989-06-24
Abstract
Abstract A diagnostic composition and method for detecting the reduction of nitrate to nitrite by micro-organisms which comprises a carrier material impregnated in specific zones with a nitratecontaining medium, a barrier composition, and two stable reagent solutions. In use, a suspension of the culture to be tested is incubated in the presence of the nitrate medium and the reduction of nitrate to nitrite is indicated by the formation of color in the reagent zones.
Inventors
- 👤 Donald P Kronish
- 👤 William D Young Jr
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 19:42
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/12; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
194 words
A diagnostic composition for the detection of nitrite, which comprises a bibulous material containing at least four impregnated zones, wherein: A. Zone A contains from about 1.4 to about 4.6 mg. of a nitrate-containing nutrient medium; B. Zone B contains a dried inert hydrophobic barrier composition, separating Zone A from all succeeding impregnated zones; C. Zone C contains 1 from about 0.005 to about 0.2 mg. of an alkali metal salt of an amino-substituted-naphthalene sulfonic acid of the group consisting of 5-amino-2-naphthalene-sulfonic acid, 8-amino-2-naphthalene-sulfonic acid, and 5-amino-1-naphthalene-sulfonic acid, and (2) from about 0.008 to about 0.36 mg. of a reagent of the group consisting of an alkali metal salt of sulfanilic acid, sulfadiazine, and an alkali metal salt of sulfathiazole; and D. Zone D contains from about 1 to about 4.5 mg. of at least one crystalline acid of the group consisting of citric acid, oxalic acid and malonic acid; said zones being arranged in an order which will promote the development of color in the reagent Zones C and D to detect any reduction of the nitrate present to nitrite by micro-organisms incubated in the presence of said nitrate-containing nutrient medium Zone A.
Claim 2
Dependent Claim
103 words
from about 0.015 to about 0.2 mg. of a reagent of the group consisting of alkali metal salt of sulfanilic acid, sulfadiazine, and an alkali metal salt of sulfathiazole; and D. Zone D contains from about 2 to about 4 mg. of at least one crystalline acid of the group consisting of citric acid, oxalic acid, and malonic acid; said zones being arranged in an order which will promote the development of color in the reagent Zones C and D to detect any reduction of the nitrate present to nitrite by micro-organisms incubated in the presence of said nitrate-containing nutrient medium Zone A.
Claim 3
Dependent Claim
123 words
about 0.08 mg. of the sodium salt of sulfanilic acid; and D. Zone D contains about 3 mg. of citric acid; wherein the zones are positioned on the bibulous material in the sequence A, B, C with Zone D applied to the bibulous material on the reverse side of Zone C; and wherein Zone A is contiguous only to Zone B; Zone B is contiguous only to Zone A and Zone C on one side of the bibulous material and contiguous only to Zone A and Zone D on the reverse side of the bibulous material; and Zone C and Zone D are positioned in a back-to-back relationship to each other and are each contiguous only to Zone B and to each other.
Claim 4
Dependent Claim
29 words
A diagnostic composition according to claim 2, wherein an additional barrier Zone B, containing a dried inert hydrophobic barrier composition, is positioned contiguous to the outermost reagent impregnated zone.
Claim 5
Dependent Claim
37 words
A diagnostic composition according to claim 3, wherein an additional Zone E, containing a dried identifying dye on one end of the diagnostic composition test strip is positioned at the end opposite the nutrient medium Zone A.
Claim 6
Dependent Claim
78 words
A diagnostic composition for the detection of nitrite, which comprises a bibulous material containing at least four reagent impregnated zones, wherein: A. Zone A contains from about 2 to about 4 mg. of a nitrate-containing nutrient medium; B. Zone B contains a sufficient amount of a dried inert hydrophobic barrier composition to saturate Zone B, and separate Zone A from all succeeding impregnated zones, said inert hydrophobic barrier composition comprising an acrylic coating composition; C. Zone C contains
Claim 7
Dependent Claim
24 words
A diagnostic composition according to claim 5, wherein an additional inert, hydrophobic barrier Zone B is positioned contiguous to the outermost reagent impregnated zone.
Claim 8
Dependent Claim
48 words
A diagnostic composition according to claim 6, wherein an additional Zone E, positioned on one end of the diagnostic composition, contains a sufficient amount of a dried identifying dye to color Zone E, said additional Zone E being positioned at the end opposite the nitrate medium Zone A.
Claim 9
Dependent Claim
84 words
A diagnostic composition for the detection of nitrite, which comprises a bibulous material containing at least four reagent impregnated zones, wherein: A. Zone A contains about 2.8 mg. of a nutrient medium prepared from about 3 mg. of beef extract, about 5 mg. of peptone and about 1 mg. of potassium nitrate; B. Zone B contains a sufficient amount of a dried inert hydrophobic barrier composition to saturate Zone B, said hydrophobic barrier composition comprising a methyl methacrylate coating composition; C. Zone C contains
Claim 10
Dependent Claim
24 words
A diagnostic composition according to claim 8, wherein an additional inert, hydrophobic barrier Zone B is positioned contiguous to the outermost reagent impregnated zone.
Claim 11
Dependent Claim
50 words
A diagnostic composition according to claim 9, wherein an additional Zone E positioned on one end of the diagnostic composition test strip, contains a sufficient amount of a dried identifying dye to color Zone E, said additional Zone E being positioned at the end opposite the nitrate medium Zone A.
Claim 12
Dependent Claim
104 words
A process for the identification and differentiation of micro-organisms which reduce nitrate to nitrite which comprises: A. Immersing Zone A of the diagnostic composition of claim 1 into a test tube containing a saline suspension of the culture to be tested; B. Incubating the test tube for about 1.5 to about 2 hours at about 35* C. to about 37* C; C. Tipping the test tube to wet Reagent Zones C and D; and D. Allowing from 30 seconds to 10 minutes to elapse for color to develop as a positive indication that the nitrate present has been reduced to nitrite by the micro-organism.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-02-28 | 1975-08-30 | 1976-02-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-02-28 | 1979-08-30 | 1980-02-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-02-28 | 1983-08-30 | 1984-02-29 | $7700.00 | Window Closed / Lapsed |