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Method of suspending immobilized biological specimens in a transparent gel in a transparent container

Assignee: Dow Chemical Co
•
Filing: 1969-12-11
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Granted: 1972-02-01
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Expires: 1989-12-11
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Abstract

Abstract A visible immobilized specimen suspended in a stable, continuous gel where the gel is a transparent adduct of a cross-linked vinyl polymer and a water-soluble aldehyde. Such immobilized specimen is prepared by providing an optically transparent container, introducing into the container a liquid phase containing a watersoluble vinyl monomer, a water-soluble aldehyde, a cross-linking agent and a free radical producing catalyst. A specimen is positioned in the liquid phase and held in position until the polymerization of the liquid phase produces sufficient gel strength to immobilize the specimen. The container is then sealed to prevent evaporation.

Inventors

  • 👤 John H Brown Jr
  • 👤 Bobby L Atkins

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-25 17:20
CPC Classifications
A; A01; A01N; A01N1/00

Claims Analysis

Total Claims: 6
Claim 1
Independent Claim 11 words
The method of claim 1 wherein the vinyl monomer is acrylamide.
Claim 2
Dependent Claim 10 words
The method of claim 1 wherein the aldehyde is formaldehyde.
Claim 3
Dependent Claim 13 words
The method of claim 1 wherein the cross-linking agent is an alkylidene bisacrylamide.
Claim 4
Dependent Claim 28 words
The method of claim 1 wherein the aqueous solution contains from about 5 to about 20 weight percent water-soluble glycol based on the total weight of the gel.
Claim 5
Dependent Claim 34 words
The method of claim 1 wherein the aldehyde is formaldehyde and wherein such formaldehyde is present in a proportion of from about 0.5 to about 50 weight percent based on the vinyl monomer present.
Claim 6
Dependent Claim 15 words
The method of claim 1 wherein the catalyst is K2S2O8 and Na2S2O5 activated with FeSO4.

References Cited (Backward Citations)

US2300495A Cited
US2486191A Cited
US2486192A Cited
US2498074A Cited
US2628178A Cited
US3153022A Cited
US3046201A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1975-01-31 1975-08-02 1976-02-01 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1979-01-31 1979-08-02 1980-02-01 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1983-01-31 1983-08-02 1984-02-01 $7700.00 Window Closed / Lapsed

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