Abstract A process for the separation of blood components, particularly of immunologically active globulins from other components, by steric chromatography, whereby plasma or serum, either in natural form or already coarsely prefractionated, is introduced into a chromatographic column which is filled with a porous auxiliary substance and is subsequently eluted from the chromatographic column with an aqueous solvent, the eluant emerging from the column being collected in fractions which may be further concentrated and/or purified. The porous auxiliary substance is a porous glass of a pore diameter of an average value of 100-250 A., said porous glass having been produced by heat treating, particulating, leaching with acid and base of the solidified product of melting together of B2O3, SiO2 and RO, wherein R is an alkali metal, alkaline earth metal or heavy metal.
The process according to claim 1 wherein the heat treatment is according to the formula: 1/Ant k exp ((-m)/T) wherein A is the internal surface area of the porous glass, t is the duration in hours of the heat treatment, T is the temperature of the heat treatment (*K.) and n, k and m are constants.
Claim 2
Dependent Claim
20 words
The process according to claim 1 wherein the heat-treated glass is comminuted to a size between 50 and 100 mesh.
Claim 3
Dependent Claim
32 words
The process according to claim 1 wherein the melt contains from 50 to 83 weight percent SiO2, from 2 to 10 weight percent RO and from 8 to 48 weight percent B2O3.
Claim 4
Dependent Claim
24 words
The process according to claim 1 wherein R is selected from Li, Na, K, Ca, Ba, Mg, Be, Sr, Zn, Pb and mixtures thereof.
Claim 5
Dependent Claim
30 words
The process according to claim 1 wherein the porous glass is prepared from a glass consisting of 6.9 weight percent Na2O, 25.7 weight percent B2O3 and 67.4 weight percent SiO2.