Patent US3701395A successfully refreshed from Google Patents.
Patent US3632475A successfully refreshed from Google Patents.
US3632475A
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Process for preparing long-chain and short-chain amyloses from starches
Assignee: Nagase Viita Co Ltd
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Filing: 1969-04-18
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Granted: 1972-01-04
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Expires: 1989-04-18
Abstract
Abstract The present invention makes it possible to separate short-chainlength amyloses and long-chain-length amyloses from starches by decomposing and cutting off the Alpha -1,6-glucoside bonds of amylopectin contained in starches by means of an Alpha -1,6glucosidase to form short-chain-length amyloses differing in molecular weight from the naturally occuring amyloses and making use of the difference in molecular weight in those two shorts of amyloses.
Inventors
- 👤 Kaname Sugimoto
- 👤 Mikihiko Yoshida
- 👤 Masashi Kurimoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:58
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/16; C08; C08B; C08B30/00; C08B30/20; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2451; C12Y; C12Y302/00; C12Y302/01; C12Y302/01033; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/831; Y10S435/832; Y10S435/847; Y10S435/848; Y10S435/853; Y10S435/859; Y10S435/863; Y10S435/866; Y10S435/874; Y10S435/882; Y10S435/885
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
30 words
A process in accordance with claim 1 in which said cooling of the liquefied starch to 45*-70* C. is done quickly and simultaneous with the addition of said Alpha -1,6-glucosidase.
Claim 2
Dependent Claim
44 words
A process in accordance with claim 2 wherein said simultaneous cooling and said addition of said Alpha -1,6-glucosidase is carried out by introducing both said gelatinized starch and said enzyme in an atomized state into a vacuum cooler to effect thorough mixing and cooling.
Claim 3
Dependent Claim
37 words
A process in accordance with claim 2 wherein said liquefied starch has a concentration of about 10 percent, and wherein after precipitation of said long-chain amylose, the remaining solution is concentrated to about 10 percent starch concentration.
Claim 4
Dependent Claim
37 words
A process in accordance with claim 2 wherein said amount of Alpha -1,6-glucosidase sufficient to decompose substantially all said amylopectin comprises about 40-50 units per gram of starch and decomposition is carried out for about 30-45 hours.
Claim 5
Dependent Claim
43 words
A process in accordance with claim 2 wherein after the amylopectin has been converted to amylose by said Alpha -1,6-glucosidase, the liquefied amylose is reheated to about 70*-90* C. prior to effecting precipitation of said long-chain amylose by cooling to about 45*-40* C.
Claim 6
Dependent Claim
55 words
A process for preparing long-chain and short-chain amyloses as defined in claim 1 in which the separation of the long-chain amyloses and short-chain amyloses is attained by adding butanol to the amylose mixture prior to cooling to 45*-40* C. and then adding additional butanol to said residual solution prior to cooling to below 5* C.
Claim 7
Dependent Claim
40 words
A process for preparing long-chain and short-chain amyloses as defined in claim 1 in which the separation of the long-chain amyloses and short-chain amyloses is attained by adding magnesium sulfate to the amylose mixture prior to cooling to 45*-40* C.
Claim 8
Dependent Claim
38 words
A process for preparing long-chain and short-chain amyloses from starches as defined in claim 1 in which the fractional precipitation of the long-chain and short-chain amyloses is promoted by subjecting the amylose mixture during cooling to ultrasonic waves.
Claim 9
Dependent Claim
30 words
A process for preparing long-chain and short-chain amyloses from starches as defined in claim 1 in which the starch is liquefied with heat in a totally enclosed, continuously operable equipment.
Claim 10
Dependent Claim
51 words
A process for preparing long-chain and short-chain aMyloses from starches as defined in claim 1 in which the Alpha -1,6-glucosidase to be used is produced by bacteria selected from the group consisting of Escherichia, Lactobacillus, Micrococcus, Nocardia, Azotobacter, Bacillus, Erwinia, Agrobacterium, Leuconostoc, Mycobacterium, Pediococcus, Sarcina, Serratia, Staphylococcus, Streptococcus, and Pseudomonas strains.
Claim 11
Dependent Claim
72 words
A process for preparing long-chain and short-chain amyloses from starches as defined in claim 11 in which, in selectively using a heat-resistant enzyme from bacteria of the genus Lactobacillus, Nocardia or the like as the Alpha -1, 6glucosidase, the slurry of liquefied starch is maintained at a temperature of 60*-70* C. and a pH value of 5-7, and the particular enzyme is added, and then the mixture is reacted at 45*-55* C.
Claim 12
Dependent Claim
39 words
A process for preparing long-chain and short-chain amyloses from starches as defined in claim 11 in which, in selectively using an enzyme from Pseudomonas bacteria as the Alpha -1,6-glucosidase, the slurry of liquefied starch is adjusted to pH 3-5.5.
References Cited (Backward Citations)
US3067067A
Cited
US3532602A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-01-03 | 1975-07-05 | 1976-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-01-03 | 1979-07-05 | 1980-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-01-03 | 1983-07-05 | 1984-01-04 | $7700.00 | Window Closed / Lapsed |