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US3625833A
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Process for producing antiviral substance from staphylococcus organisms
Assignee: JAMES J SCHAFFER
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Filing: 1970-01-23
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Granted: 1971-12-07
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Expires: 1990-01-23
Abstract
Abstract Staphylococcus bacteria are cultured to produce an antiviral substance having inhibitory activity against Coxsacki B virus, Semliki-Forest virus, and the like. For use in the process, a nonpathogenic strain of staphylococcus bacteria is selected, the strain being classifiable as Staphylococcus albus or Staphylococcus aureus (albus variant). The strain is propagated in a culture medium containing a growth-promoting concentration of aspartic acid, preferably DL aspartic acid. The antiviral substance which can be recovered from the broth has utility in treating viral infections of animals, including transmissible gastroenteritis (TGE) in swine and distemper in dogs and cats.
Inventors
- 👤 James J Schaffer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 19:51
CPC Classifications
C; C12; C12N; C12N9/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/882; Y10S435/883
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
24 words
The process steps of claim 1 where said aspartic acid is added to said culture medium as a mixture of its D- and L-forms.
Claim 2
Dependent Claim
19 words
The process steps of claim 1 in which said aspartic acid is primarily in the form of L-aspartic acid.
Claim 3
Dependent Claim
45 words
The process steps of claim 1 where said aspartic acid is in the form of a D-, L-racemic mixture thereof and is employed at a concentration within the range from about 8 to 25 grams of said racemic mixture per liter of said culture medium.
Claim 4
Dependent Claim
109 words
In the process of culturing of viable staphylococcus bacteria to produce an antiviral substance having inhibitory activity against Coxsacki B virus, Semliki-Forest virus, and the like, the process steps of: a. selecting a nonpathogenic strain of staphylococcus bacteria producing said substance, said staphylococcus being classifiable as Staphylococcus albus or Staphylococcus aureus (albus variant), and being further characterized as nonhemolytic, coagulose-negative, mannite-negative, and penicillin-sensitive; b. preparing an aqueous culture medium for said staphylococcus bacteria and incorporating therein from 5 to 30 grams per liter of a D-, L-mixture of aspartic acid; and c. propagating said staphylococcus bacteria in said aspartic acid-containing culture medium to produce said substance in enhanced quantities.
Claim 5
Dependent Claim
23 words
The process steps of claim 5 in which said aspartic acid is employed at a concentration of about 8- 25 grams per liter.
Claim 6
Dependent Claim
106 words
In the process of culturing of viable staphylococcus bacteria to produce an antiviral substance having inhibitory activity against Coxsacki B virus, Semliki-Forest virus, and the like, the process steps of: a. selecting a nonpathogenic strain of staphylococcus bacteria producing said substance, said staphylococcus being classifiable as Staphylococcus albus or Staphylococcus aureus (albus variant) and being further characterized as nonhemolytic, coagulose-negative, mannite-negative, and penicillin-sensitive; b. preparing an aqueous culture medium for said staphylococcus bacteria and incorporating therein from about 2.5 to 15 grams per liter of L-aspartic acid; and c. propagating said staphylococcus bacteria in said aspartic acid-containing culture medium to produce said substance in enhanced quantities.
References Cited (Backward Citations)
US3303094A
Cited
US3398056A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-12-06 | 1975-06-07 | 1975-12-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-12-06 | 1979-06-07 | 1979-12-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-12-06 | 1983-06-07 | 1983-12-07 | $7700.00 | Window Closed / Lapsed |