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US3619371A
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Production of a polymeric matrix having a biologically active substance bound thereto
Assignee: NAT RES DEV
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Filing: 1968-06-26
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Granted: 1971-11-09
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Expires: 1988-06-26
Abstract
Abstract A polymeric matrix having a biologically active substance chemically bound thereto, which comprises a polymer and a biologically active substance linked by groups of the formula: WHERE Y represents a nucleophilic substituent that is an amino group, or an aliphatic or aromatic group.
Inventors
- 👤 Eric Mitchell Crook
- 👤 Garth Kay
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:51
CPC Classifications
C; C08; C08B; C08B15/00; C08B15/05; C08B15/06; C12; C12H; C12H1/00; C12H1/003; C12N; C12N11/00; C12N11/02; C12N11/06; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
32 words
A polymeric matrix according to claim 1 in which the group Y is one that carries a positive charge when in contact with solutions having a pH in the normal biological range.
Claim 2
Dependent Claim
31 words
A polymeric matrix according to claim 2 in which the group Y is one that carries a positive charge when in contact with solutions having a pH between 5 and 9.
Claim 3
Dependent Claim
20 words
A polymeric matrix according to claim 1 in which the group Y is one that contains nitrogen, oxygen or sulfur.
Claim 4
Dependent Claim
34 words
A polymeric matrix according to claim 1, in which the group Y is an amino, alkyl or aryl substituted amino, alkylamino, arylamino, oxyalkyl, oxyaryl, thioalkyl or thioaryl group, or a residue of a dyestuff.
Claim 5
Dependent Claim
25 words
A polymeric matrix according to claim 1, in which the group Y is -O.CH2C00H, -NH.CH2C00H, -S.CH2C00H, -NH.C2H4.SO3H, -OC4H8N (C2H5)3, -NH.C6H4SO3H, -O.C6H4C00H, -S.C6H4C00H, -NH.C2H4.OH, or -O.C2H4.OH.
Claim 6
Dependent Claim
15 words
A polymeric matrix according to claim 1, in which the group Y is -NC3H6NH (C2H40H)2.
Claim 7
Dependent Claim
18 words
A polymeric matrix according to claim 1, in which the polymer is one that has free hydroxyl groups.
Claim 8
Dependent Claim
24 words
A polymer matrix according to claim 8, in which the polymer is a cellulose, a cross-linked dextron, starch, dextran, a protein or polyvinyl alcohol.
Claim 9
Dependent Claim
28 words
A polymeric matrix according to claim 1, in which the biologically active substance is a proteolytic enzyme, a hydrolase, a dehydrogenase, a kinase, an oxidase, or an amidase.
Claim 10
Dependent Claim
15 words
A polymeric matrix according to claim 1, in which the biologically active substance is chymotrypsin.
Claim 11
Dependent Claim
27 words
A polymeric matrix according to claim 1, in which the biologically active substance is lactic dehydrogenase, Beta -galactosidase, creatine phospho-kinase, pyruvate kinase, an antigen, or an antibody.
Claim 12
Dependent Claim
28 words
A polymeric matrix according to claim 1, in which the biologically active substance is trypsin, papain, ribonuclease, alkaline phosphotase, amylo glucosidase, dextranase, glucose oxidase, amidase, or penicillin amidase.
Claim 13
Dependent Claim
50 words
A process of producing a polymeric matrix having a biologically active substance selected from the group consisting of enzymes present in or isolated from animal, plant or microbiological tissue, antigens and antibodies, chemically bound thereto, which comprises reacting the substance with a polymer having attached thereto groups of the formula:
Claim 14
Dependent Claim
13 words
A process according to claim 14, in which X represents a halogen atom.
Claim 15
Dependent Claim
14 words
A process according to claim 15, in which the halogen is chlorine or fluorine.
Claim 16
Dependent Claim
14 words
A process according to claim 14, that is carried out in an aqueous medium.
Claim 17
Dependent Claim
15 words
A process according to claim 17, that is carried out at a slightly alkaline pH.
Claim 18
Dependent Claim
21 words
A process according to claim 14, in which any radicals X remaining after reaction with the biologically active substance are deactivated.
Claim 19
Dependent Claim
21 words
A process according to claim 19, in which the radicals X are deactivated by reaction with ammonia or an aliphatic amine.
References Cited (Backward Citations)
US3403146A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-11-08 | 1975-05-10 | 1975-11-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-11-08 | 1979-05-10 | 1979-11-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-11-08 | 1983-05-10 | 1983-11-09 | $7700.00 | Window Closed / Lapsed |