Patent US3616455A successfully refreshed from Google Patents.
US3616455A
Expired / Lapsed
US
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Electrophoretic separation apparatus
Assignee: Individual
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Filing: 1969-03-13
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Granted: 1971-10-26
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Expires: 1989-03-13
Abstract
Abstract An electrophoretic separation apparatus for the support-free continuous separation of molecular fractions in a buffer solution has a thin flat electrophoresis chamber defined between watercooled plates through which the mixture of fractions and buffer solution is passed and wherein the solution is subjected to a unidirectional field transverse to the direction of mass flow to separate the fractions. Subsequently the separated fractions are sedimented out of their respective portions of buffer solution in collecting vessels ahead of the outlets for the solution. Downstream of the collecting vessels is mechanical or hydrostatic pump or flow-control means which makes the fluid flow through the apparatus.
Inventors
- 👤 Philipp Adolf Von Munchhausen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 17:23
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44769
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
47 words
The apparatus defined in claim 1, further comprising conduits connecting said collecting means to said pump means and to said chamber, said collecting means including a collecting vessel having a cross-sectional area substantially greater than the cross-sectional diameter of said conduits to permit sedimentation in said vessel.
Claim 2
Dependent Claim
54 words
The apparatus defined in claim 1, further comprising a pair of spaced parallel plates forming said chamber between them and cooling means for preventing overheating of said plates, said outlet comprising a plurality of outlet openings spaced along a wall of said chamber, said inlet being formed along an opposite wall of said chamber.
Claim 3
Dependent Claim
27 words
The apparatus defined in claim 3, further comprising means for tilting said plates jointly through substantially 90* from a substantially vertical position to a substantially horizontal position.
Claim 4
Dependent Claim
59 words
The apparatus defined in claim 1 wherein said collecting means comprises a substantially vertical burette connected at a lower end thereof to said outlet, and means connecting said burette to said pump means at an upper end of said burette, said burette being provided at said lower end with means for draining off said one of said sedimented fractions.
Claim 5
Dependent Claim
33 words
The apparatus defined in claim 1 wherein said pump means comprises a peristaltic pump, said apparatus further comprising a flexible conduit attached to said collecting means, said pump coacting with said flexible conduit.
Claim 6
Dependent Claim
26 words
The apparatus defined in claim 1 wherein said collecting means comprises a capillary tube, said pump means comprising means for maiNtaining a head in said tube.
Claim 7
Dependent Claim
23 words
The apparatus defined in claim 1, further comprising valve means between said outlet and said collecting means for selectively prohibiting fluid flow therebetween.
Claim 8
Independent Claim
150 words
An apparatus for the continuous electrophoretic separation of molecular fractions in a buffer solution, said apparatus comprising: a support; a pair of spaced-apart liquid-cooled plates mounted on said support and defining between them a narrow flat separation chamber having an inlet side and an outlet side opposite one another whereby a mass flow of the buffer solution and mixture of fractions traverse said chamber from said inlet side to said outlet side; means for applying a generally unidirectional field across the mass flow through said chamber to separate said fractions such that they arrive at different locations along said outlet side; means at said outlet side for abstracting said fractions from said chamber at said locations; and tilting means between said support and said plates enabling tilting of said chamber about a substantially horizontal axis through an angle of about 90*, and for retaining said chamber in said tilted position.
Claim 9
Dependent Claim
50 words
The apparatus defined in claim 9 wherein said plates and said chamber are generally rectangular and said sides are horizontal, said tilting means including a hinge connected to said plates at said outlet side of said chamber enabling swinging of said chamber between a recumbent position and an upright position.
Claim 10
Dependent Claim
125 words
The apparatus defined in claim 10 wherein said means for abstracting said fractions from said chamber at said outlet side includes: a respective flexible tube leading from said chamber and communicating therewith at each of said locations; a respective upright elongated sedimentation vessel connected with each of said tubes and communicating therewith at a location above the bottom of the respective vessel; means at the bottom of each of said vessels for draining same; means at an upper portion of each of said vessels above the location at which the respective tube communicates therewith for inducing a flow of said solution through the corresponding tube and vessel whereby said fractions are sedimented at the bottom of said vessels; and means for selectively closing said tubes.
Claim 11
Dependent Claim
27 words
The apparatus defined in claim 11 wherein said vessels are burettes having valves at their bottoms, and said means for selectively closing said tubes are pinch clamps.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-10-25 | 1975-04-26 | 1975-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-10-25 | 1979-04-26 | 1979-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-10-25 | 1983-04-26 | 1983-10-26 | $7700.00 | Window Closed / Lapsed |