Precolumn inlet for chromatographs

Abstract

Abstract Removable precolumn inlet apparatus for direct injection into high resolution packed or capillary columns for chromatographs. A front body and a rear body, the front body being removably attached to the rear body and contains a hot zone where evaporation of samples occur and an air-cooled zone in which sealing septums are disposed. A precolumn tube is disposed in the front body which is heated so that samples are evaporated in said tube. A separatory column of high revolving ability is operably connected to the precolumn tube, said rear body having a cavity in which a short length of the high-resolution column extends, said short length of the high-resolution column being refrigerated, there being a cold shield in the cavity to reduce the heat loss of the rear body when refrigeration is taking place. The rear body also acts as a heat source for rapidly warming up the cold zone after the sample components have been condensed on the head end of the column. The short linear dimension of the high-resolution column is subjected to refrigeration in the cavity of the rear body so as to condense the sample components and thereby provide a sharp sample injection into the continuing high-resolution column when refrigeration is turned off. The surrounding heat block of the rear body provides a large thermal mass to rapidly bring the capillary column segment back up to column temperature.

Inventors

• 👤 Carol A M G Cramers
• 👤 Mateus M Van Kessel

Bibliographic Details