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US3572892A Expired / Lapsed US ⏱️ 0.0 years remaining

Multiple well tissue culture slide

Assignee: Richardson Vicks Inc
•
Filing: 1969-02-10
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Granted: 1971-03-30
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Expires: 1989-02-10
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Abstract

Abstract A tissue culture microscope slide comprising a transparent flat plate having depressions or wells on the top surface thereof. The walls defining each well have an opening extending from the top to the bottom of the well adjacent to an edge of the plate to facilitate the drainage of liquids therefrom. The bottom surface of each well is substantially parallel with the bottom surface of the plate. The tissue culture slide after preparation with fixed, confluent, viral infected tissue culture cells can be used with fluorescent labeled reagents and a fluorescent microscope to provide the technologist or clinician with a tool for rapidly screening the sera of patients or animals for a variety of viral agents as measured by the presence in the patients'' or animals'' serum of antibody against a specific viral agent.

Inventors

  • 👤 Don P Metzgar
  • 👤 James V Sorrentino

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-03 11:08
CPC Classifications
C; C12; C12M; C12M41/00; C12M41/46; C12M23/00; C12M23/02; C12M23/04; C12M23/12; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G02; G02B; G02B21/00; G02B21/34

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 75 words
A multiple well tissue culture microscope slide comprising: a. a flat plate of transparent material; b. a plurality of wells or depressions on the top surface of the plate; c. said wells having an opening on one side thereof adjacent an edge of said plate, said opening extending from top to bottom of the well to facilitate drainage of liquids therefrom; and wherein d. the bottom surface of said plate opposite each well is flat.
Claim 2
Dependent Claim 75 words
A multiple well tissue culture microscope slide comprising: a. a flat plate of transparent material; b. a plurality of wells or depressions on the top surface of the plate; c. said wells having an opening on one side thereof adjacent an edge of said plate, said opening extending from top to bottom of the well to facilitate drainage of liquids therefrom; and wherein d. the bottom surface of said plate opposite each well is flat.
Claim 3
Dependent Claim 93 words
A multiple well tissue culture microscope slide comprising: a. a flat plate of transparent material; b. a series of depressions or wells spaced from each other on the top surface of said plate; c. the bottoms of said depressions being flat and in a plane parallel with the bottom of the slide; d. each of said depressions defined by sidewalls and a bottom; e. said sidewalls having an opening adjacent one of the plate edges, said opening extending from the top to the bottom of said wells to facilitate drainage of liquids therefrom.
Claim 4
Dependent Claim 93 words
A multiple well tissue culture microscope slide comprising: a. a flat plate of transparent material; b. a series of depressions or wells spaced from each other on the top surface of said plate; c. the bottoms of said depressions being flat and in a plane parallel with the bottom of the slide; d. each of said depressions defined by sidewalls and a bottom; e. said sidewalls having an opening adjacent one of the plate edges, said opening extending from the top to the bottom of said wells to facilitate drainage of liquids therefrom.
Claim 5
Dependent Claim 106 words
A multiple well tissue culture microscope slide comprising: a. a flat plate of transparent material; b. ribs projecting upwardly on the top surface of said plate as an integral part thereof defining a series of wells spaced from each other; c. said ribs enclosing a major portion of the wells and having an opening extending from the top to the bottom of the wells adjacent a plate edge to permit fluid communication between the well and said plate edge to facilitate drainage of liquids therefrom; and d. wherein the bottom surface of said wells is substantially flat and parallel to the bottom surface of said plate.
Claim 6
Dependent Claim 106 words
A multiple well tissue culture microscope slide comprising: a. a flat plate of transparent material; b. ribs projecting upwardly on the top surface of said plate as an integral part thereof defining a series of wells spaced from each other; c. said ribs enclosing a major portion of the wells and having an opening extending from the top to the bottom of the wells adjacent a plate edge to permit fluid communication between the well and said plate edge to facilitate drainage of liquids therefrom; and d. wherein the bottom surface of said wells is substantially flat and parallel to the bottom surface of said plate.
Claim 7
Dependent Claim 109 words
A multiple well tissue culture microscope slide comprising: a. a transparent, flat rectangular plate; b. a first row of depressions or wells on the top surface of said plate adjacent one of the plate long sides; c. a second row of depressions or wells on the top surface of said plate adjacent the other long side of the plate; d. the bottom surface of the wells being substantially flat and parallel with the plate bottom; and e. each row of wells having an opening extending from the bottom surface thereof to the top facing the adjacent plate edge, said openings facilitating the drainage of fluid out of said wells.
Claim 8
Dependent Claim 109 words
A multiple well tissue culture microscope slide comprising: a. a transparent, flat rectangular plate; b. a first row of depressions or wells on the top surface of said plate adjacent one of the plate long sides; c. a second row of depressions or wells on the top surface of said plate adjacent the other long side of the plate; d. the bottom surface of the wells being substantially flat and parallel with the plate bottom; and e. each row of wells having an opening extending from the bottom surface thereof to the top facing the adjacent plate edge, said openings facilitating the drainage of fluid out of said wells.
Claim 9
Dependent Claim 27 words
A multiple well tissue culture slide of claim 4 having attached to the bottom surface of said wells a fixed, confluent sheet of cells infected with virus.

References Cited (Backward Citations)

US1516896A Cited
US2281617A Cited
US2302830A Cited
US3031924A Cited
US3432275A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1974-03-29 1974-09-28 1975-03-30 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1978-03-29 1978-09-28 1979-03-30 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1982-03-29 1982-09-28 1983-03-30 $7700.00 Window Closed / Lapsed

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