Patent US3560754A successfully refreshed from Google Patents.
US3560754A
Expired / Lapsed
US
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Photoelectric particle separator using time delay
Assignee: International Business Machines Corp
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Filing: 1965-11-17
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Granted: 1971-02-02
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Expires: 1985-11-17
Abstract
Abstract This disclosure relates to apparatus for discriminating between cancer and normal cells by measuring the absorption by nucleic acids per unit volume of each cell in a sample and separating the cancer cells from the sample by shunting the cancer cells from a main flow channel into a shunting junction. Upon being presented at the junction, after a suitable delay, a force is applied at the junction to the cancer cell and the cell is passed into the shunting junction for subsequent collection. The force applied to the cell may be hydraulic, pneumatic, electrostatic or some combination of these forces.
Inventors
- 👤 Louis A Kamentsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:12
CPC Classifications
B; B07; B07C; B07C5/00; B07C5/34; B07C5/342; G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1456; G01N15/1459; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491; G01N15/149; Y; Y10; Y10S; Y10S209/00; Y10S209/906
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
94 words
Apparatus for separating discrete microscopic cellular bodies disposed in a liquid medium according to at least a desired characteristic comprising in combination: a source of said discrete cellular bodies disposed in a carrier liquid, means for transporting said discrete cellular bodies seriatim along a given path under pressure, means disposed in juxtaposition with said first mentioned means for detecting a desired characteristic of said discrete cellular bodies and means responsive to said detecting means for removing selected portions of said carrier liquid each containing at least a discrete cellular body from said given path.
Claim 2
Dependent Claim
94 words
Apparatus for separating discrete microscopic cellular bodies disposed in a liquid medium according to at least a desired characteristic comprising in combination: a source of said discrete cellular bodies disposed in a carrier liquid, means for transporting said discrete cellular bodies seriatim along a given path under pressure, means disposed in juxtaposition with said first mentioned means for detecting a desired characteristic of said discrete cellular bodies and means responsive to said detecting means for removing selected portions of said carrier liquid each containing at least a discrete cellular body from said given path.
Claim 3
Dependent Claim
20 words
Apparatus according to claim 1 further including a junction which divides said given path into at least two separate channels.
Claim 4
Dependent Claim
35 words
Apparatus according to claim 1 further including means for introducing at least a predetermined delay interposed between said detection means and said means for removing selected portions of said carrier liquid from said given path.
Claim 5
Dependent Claim
30 words
Apparatus according to claim 1 further including variable delay means interposed between said detection means and said means for removing selected portions of said carrier liquid from said given path.
Claim 6
Dependent Claim
24 words
Apparatus according to claim 1 further including means disposed in juxtaposition to said given path for collecting said selected portions of said carrier liquid.
Claim 7
Dependent Claim
33 words
Apparatus according to claim 1 further including means for applying a positive pressure to said carrier liquid containing said discrete cellular bodies coupled to said first mentioned means at least another extremity thereof.
Claim 8
Dependent Claim
28 words
Apparatus according to claim 6 further including means for applying a pressure less than said positive pressure to said first mentioned means at at least another extremity thereof.
Claim 9
Dependent Claim
14 words
Apparatus according to claim 1 wherein said means for transporting includes a capillary tube.
Claim 10
Dependent Claim
34 words
Apparatus according to claim 1 wherein said means for transporting include a member having a channel disposed therein of a size insufficient to pass more than one of said discrete bodies at any instant.
Claim 11
Dependent Claim
54 words
Apparatus according to claim 1 wherein said means for detecting a desired characteristic includes optical means adapted to provide at least a signal output in response to the absorption characteristic of said discrete bodies at a given wavelength and to the light scattering characteristic of said discrete bodies at wavelengths including said given wavelength.
Claim 12
Dependent Claim
44 words
Apparatus according to claim 1 wherein said means responsive to said detection means for removing selected portions of said carrier liquid from said given path includes at least valving means disposed in at least a path apart from and connected to said given path.
Claim 13
Dependent Claim
50 words
Apparatus according to claim 1 wherein said means responsive to said detection means for removing selected portions of said carrier fluid from said given path includes valving means disposed in said given path and valving means disposed in at least a path apart from and connected to said given path.
Claim 14
Dependent Claim
17 words
Apparatus according to claim 12 further including a source of suction connected to each said valving means.
Claim 15
Dependent Claim
31 words
Apparatus according to claim 12 further including means for intermittently actuating said valving means whereby suction is alternately applied to said given path and said path apart from said given path.
Claim 16
Dependent Claim
45 words
Apparatus according to claim 14 wherein said means for actuating said valving means includes translating means coupled to said detecting means adapted to provide at least an electrical output and at least an amplifier adapted to provide an augmented output to actuate said valving means.
Claim 17
Dependent Claim
11 words
Apparatus according to claim 15 wherein translating means includes a photocell.
Claim 18
Dependent Claim
93 words
Apparatus for separating discrete cellular bodies held in a carried liquid comprising in combination: a source of said discrete cellular bodies disposed in a carried liquid, means for detecting at least a desired characteristic of said bodies, a shunting junction, means for presenting said bodies to said detecting means and said shunting junction, and means responsive to said detecting means for applying a force intermittently to said carrier liquid at said junction to cause selected portions of said carrier liquid each containing at least one of said bodies to enter said shunting junction.
Claim 19
Dependent Claim
93 words
Apparatus for separating discrete cellular bodies held in a carried liquid comprising in combination: a source of said discrete cellular bodies disposed in a carried liquid, means for detecting at least a desired characteristic of said bodies, a shunting junction, means for presenting said bodies to said detecting means and said shunting junction, and means responsive to said detecting means for applying a force intermittently to said carrier liquid at said junction to cause selected portions of said carrier liquid each containing at least one of said bodies to enter said shunting junction.
Claim 20
Dependent Claim
46 words
Apparatus according to claim 17 wherein said means for applying a force to said carrier liquid includes at least a source of suction and actuable means for intermittently applying said source of suction at said junction to shunt selected of said discrete bodies from said junction.
Claim 21
Dependent Claim
13 words
Apparatus according to claim 1 wherein said discrete bodies are microscopic in size.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-02-01 | 1974-08-03 | 1975-02-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-02-01 | 1978-08-03 | 1979-02-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-02-01 | 1982-08-03 | 1983-02-02 | $7700.00 | Window Closed / Lapsed |