Patent US3337529A successfully refreshed from Google Patents.
US3337529A
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Method for chromatographically separating nucleotides and other organic materials from a mixture containing same
Assignee: SCHWARZ BIORES Inc
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Filing: 1965-05-18
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Granted: 1967-08-22
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Expires: 1985-05-18
Abstract
No abstract record available.
Inventors
- 👤 Laufer Louis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 20:23
CPC Classifications
C; C07; C07H; C07H1/00; C07H1/06; B; B01; B01J; B01J47/00; B01J47/014
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
127 words
A METHOD FOR FRACTIONATING A MIXTURE CONTAINING NUCLEOSIDE PHOSPHATE ESTERS COMPRISING THYMIDINE TRIPHOSPHATE, ADENOSINE TRIPHOSPHATE, RELATED MONO- AND DI-PHOSPHATES AND INORGANIC SALTS, WHICH COMPRISES: (A) SUBJECTING SAID MIXTURE TO CHROMATOGRAPHY ON A VERTICAL COLUMN OF A SYNTHETIC ANION EXCHANGE RESIN IN THE CHLORIDE FORM TO FORM AT LEAST TWO CHROMATOGRAPHIC BANDS ON SAID RESIN; (B) ELUTING THE COLUMN IN (A) WITH WEAK ACID TO REMOVE SAID MONO-AND DI-PHOSPHATES; (C) WASHING SAID RESIN WITH WATER UNTIL AN ELUATE WITHDRAWN IS CHLORIDE FREE; (D) INVERTING SAID COLUMN; (E) ELUTING THE COLUMN OF (D) WITH AQUEOUS AMMONIUM BICARBONATE; AND (F) RECOVERING A FIRST ELUATE CONTAINING THYMIDINE TRIPHOSPHATE SUBSTANTIALLY FREE OF OTHER COMPOUNDS OF SAID MIXTURE, AND A SECOND ELUATE CONTAINING ADENOSINE TRIPHOSPHATE SUBSTANTIALLY FREE OF OTHER COMPOUNDS OF SAID MIXTURE.
Claim 2
Independent Claim
127 words
A METHOD FOR FRACTIONATING A MIXTURE CONTAINING NUCLEOSIDE PHOSPHATE ESTERS COMPRISING THYMIDINE TRIPHOSPHATE, ADENOSINE TRIPHOSPHATE, RELATED MONO- AND DI-PHOSPHATES AND INORGANIC SALTS, WHICH COMPRISES: (A) SUBJECTING SAID MIXTURE TO CHROMATOGRAPHY ON A VERTICAL COLUMN OF A SYNTHETIC ANION EXCHANGE RESIN IN THE CHLORIDE FORM TO FORM AT LEAST TWO CHROMATOGRAPHIC BANDS ON SAID RESIN; (B) ELUTING THE COLUMN IN (A) WITH WEAK ACID TO REMOVE SAID MONO-AND DI-PHOSPHATES; (C) WASHING SAID RESIN WITH WATER UNTIL AN ELUATE WITHDRAWN IS CHLORIDE FREE; (D) INVERTING SAID COLUMN; (E) ELUTING THE COLUMN OF (D) WITH AQUEOUS AMMONIUM BICARBONATE; AND (F) RECOVERING A FIRST ELUATE CONTAINING THYMIDINE TRIPHOSPHATE SUBSTANTIALLY FREE OF OTHER COMPOUNDS OF SAID MIXTURE, AND A SECOND ELUATE CONTAINING ADENOSINE TRIPHOSPHATE SUBSTANTIALLY FREE OF OTHER COMPOUNDS OF SAID MIXTURE.
References Cited (Backward Citations)
US3148139A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1970-08-21 | 1971-02-20 | 1971-08-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1974-08-21 | 1975-02-20 | 1975-08-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1978-08-21 | 1979-02-20 | 1979-08-22 | $7700.00 | Window Closed / Lapsed |