US3275528A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for controlling culture growth
Assignee: California Institute Research Foundation
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Filing: 1961-03-30
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Granted: 1966-09-27
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Expires: 1981-03-30
Abstract
No abstract record available.
Inventors
- 👤 Ainis Herman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:21
CPC Classifications
C; C12; C12M; C12M29/00; C12M29/04; C12M23/00; C12M23/02; C12M23/08; C12M23/10; C12M23/34
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
163 words
AN APPARATUS FOR CONTROLLING CULTURE GROWTH COMPRISING A HOUSING INCLUDING A GAS CHAMBER, FIRST FLUID CHAMBER POSITIONED ADJACENT TO SAID GAS CHAMBER, A SECOND FLUID CHAMBER POSITIONED ADJACENT TO SAID FIRST FLUID CHAMBER, MEANS FOR CIRCULATING GAS IN AND THROUGH SAID GAS CHAMBER, MEANS FOR CIRCULATING FLUID IN AND THROUGH SAID FIRST FLUID CHAMBER, MEANS FOR CIRCULATING FLUID THROUGH SAID SECOND FLUID CHAMBER, A FIRST POROUS MEANS POSITIONED IN SAID FIRST FLUID CHAMBER AND SEPARATING SAID GAS CHAMBER FROM SAID FIRST FLUID CHAMBER, SAID FIRST POROUS MEANS HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF THAT INSULIN BUT INSUFFICIENT TO PASS CELLS, AND A SECOND POROUS MEANS POSITIONED IN SAID SECOND FLUID CHAMBER AND SEPARATING SAID SECOND FLUID CHAMBER FROM SAID FIRST FLUID CHAMBER, SAID POROUS MEANS HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF UREA, BUT INSUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF INSULIN.
Claim 2
Independent Claim
163 words
AN APPARATUS FOR CONTROLLING CULTURE GROWTH COMPRISING A HOUSING INCLUDING A GAS CHAMBER, FIRST FLUID CHAMBER POSITIONED ADJACENT TO SAID GAS CHAMBER, A SECOND FLUID CHAMBER POSITIONED ADJACENT TO SAID FIRST FLUID CHAMBER, MEANS FOR CIRCULATING GAS IN AND THROUGH SAID GAS CHAMBER, MEANS FOR CIRCULATING FLUID IN AND THROUGH SAID FIRST FLUID CHAMBER, MEANS FOR CIRCULATING FLUID THROUGH SAID SECOND FLUID CHAMBER, A FIRST POROUS MEANS POSITIONED IN SAID FIRST FLUID CHAMBER AND SEPARATING SAID GAS CHAMBER FROM SAID FIRST FLUID CHAMBER, SAID FIRST POROUS MEANS HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF THAT INSULIN BUT INSUFFICIENT TO PASS CELLS, AND A SECOND POROUS MEANS POSITIONED IN SAID SECOND FLUID CHAMBER AND SEPARATING SAID SECOND FLUID CHAMBER FROM SAID FIRST FLUID CHAMBER, SAID POROUS MEANS HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF UREA, BUT INSUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF INSULIN.
Claim 3
Independent Claim
208 words
A METHOD OF CONTROLLING TISSUE GROWTH IN AN APPARATUS HAVING A GAS CHAMBER, A FIRST FLUID CHAMBER AND A SECOND FLUID CHAMBER, SAID FIRST FLUID CHAMBER BEING PROVIDED WITH A POROUS MEANS SEPARATING SAID GAS CHAMBER FROM SAID FIRST FLUID CHAMBER AND HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF INSULIN, BUT INSUFFICIENT TO PASS CELLS, SAID SECOND FLUID CHAMBER BEING PROVIDED WITH A POROUS MEANS SEPARATING SAID FIRST FLUID CHAMBER FROM SAID SECOND FLUID CHAMBER AND HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF UREA, BUT INSUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF INSULIN, COMPRISING THE STEPS OF PLACING EXPLANTED CELLS IN SAID FIRST FLUID CHAMBER, SUBJECTING SAID CELLS TO A GASEOUS ATMOSPHERE COMPATIBLE THEREWITH, SLOWLY PASSING A NUTRIENT MEDIUM THROUGH SAID FIRST FLUID CHAMBER AND BATHING SAID CELLS WITH SAID MEDIUM, SLOWLY PASSING A LIKE MEDIUM THROUGH SAID SECOND FLUID CHAMBER WHEREBY BY-PRODUCTS HAVING A SIZE SUFFICIENTLY SMALL TO PASS THROUGH SAID SECOND POROUS MEANS ARE REMOVED FROM SAID FIRST FLUID CHAMBER, AND COLLECTING MEDIUM PASSED THROUGH SAID FIRST FLUID CHAMBER WHEREBY CELL BY-PRODUCTS HAVING A SIZE TOO LARGE TO PASS THROUGH SAID SECOND POROUS MEANS ARE OBTAINED.
Claim 4
Independent Claim
208 words
A METHOD OF CONTROLLING TISSUE GROWTH IN AN APPARATUS HAVING A GAS CHAMBER, A FIRST FLUID CHAMBER AND A SECOND FLUID CHAMBER, SAID FIRST FLUID CHAMBER BEING PROVIDED WITH A POROUS MEANS SEPARATING SAID GAS CHAMBER FROM SAID FIRST FLUID CHAMBER AND HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF INSULIN, BUT INSUFFICIENT TO PASS CELLS, SAID SECOND FLUID CHAMBER BEING PROVIDED WITH A POROUS MEANS SEPARATING SAID FIRST FLUID CHAMBER FROM SAID SECOND FLUID CHAMBER AND HAVING A PORE SIZE SUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF UREA, BUT INSUFFICIENT TO PASS MOLECULES HAVING A SIZE ON THE ORDER OF INSULIN, COMPRISING THE STEPS OF PLACING EXPLANTED CELLS IN SAID FIRST FLUID CHAMBER, SUBJECTING SAID CELLS TO A GASEOUS ATMOSPHERE COMPATIBLE THEREWITH, SLOWLY PASSING A NUTRIENT MEDIUM THROUGH SAID FIRST FLUID CHAMBER AND BATHING SAID CELLS WITH SAID MEDIUM, SLOWLY PASSING A LIKE MEDIUM THROUGH SAID SECOND FLUID CHAMBER WHEREBY BY-PRODUCTS HAVING A SIZE SUFFICIENTLY SMALL TO PASS THROUGH SAID SECOND POROUS MEANS ARE REMOVED FROM SAID FIRST FLUID CHAMBER, AND COLLECTING MEDIUM PASSED THROUGH SAID FIRST FLUID CHAMBER WHEREBY CELL BY-PRODUCTS HAVING A SIZE TOO LARGE TO PASS THROUGH SAID SECOND POROUS MEANS ARE OBTAINED.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1969-09-26 | 1970-03-28 | 1970-09-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1973-09-26 | 1974-03-28 | 1974-09-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1977-09-26 | 1978-03-28 | 1978-09-27 | $7700.00 | Window Closed / Lapsed |