US2990335A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluid suspension culture method for fixed tissue cells
Assignee: Individual
•
Filing: 1955-05-06
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Granted: 1961-06-27
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Expires: 1975-05-06
Abstract
No abstract record available.
Inventors
- 👤 Wilton R Earle
- 👤 Virginia J Evans
- 👤 Edward L Schilling
- 👤 Jay C Bryant
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 01:34
CPC Classifications
C; C12; C12M; C12M27/00; C12M27/02; C12M23/00; C12M23/02; C12M23/08; C12M41/00; C12M41/30; C12M41/34
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
100 words
A METHOD OF PROLIFERATION OF ANIMAL FIXED TISSUE CELLS IN SUSPENSION THAT COMPRISES PREPARING A BODY OF LIQUID CULTURE MEDIUM CONTAINING CELL NUTRIENT IN SOLUTION, INOCULATING SAID BODY WITH ANIMAL FIXED TISSUE CELLS PRESUSPENDED IN CULTURE MEDIUM IN A QUANTITY PRODUCING A POPULATION OF THE ORDER OF 400,000 TO 1,000,000 CELLS PER MILLILITER OF THE RESULTING CULTURE, MAINTAINING THE CULTURE AT A TEMPERATURE OF ABOUT 37.5*C. AND APPROXIMATELY ATMOSPHERIC PRESSURE, AND SUBSTANTIALLY CONTINUOUSLY AGITATING THE INOCULATED CULTURE MEDIUM BY SWIRLING IT ABOUT IN A CONTAINER WHILE CIRCULATING IN CONTACT THEREWITH AN ATMOSPHERE CONTINUOUSLY MAINTAINED AT APPROXIMATELY 5% CO2 IN AIR.
Claim 2
Independent Claim
100 words
A METHOD OF PROLIFERATION OF ANIMAL FIXED TISSUE CELLS IN SUSPENSION THAT COMPRISES PREPARING A BODY OF LIQUID CULTURE MEDIUM CONTAINING CELL NUTRIENT IN SOLUTION, INOCULATING SAID BODY WITH ANIMAL FIXED TISSUE CELLS PRESUSPENDED IN CULTURE MEDIUM IN A QUANTITY PRODUCING A POPULATION OF THE ORDER OF 400,000 TO 1,000,000 CELLS PER MILLILITER OF THE RESULTING CULTURE, MAINTAINING THE CULTURE AT A TEMPERATURE OF ABOUT 37.5*C. AND APPROXIMATELY ATMOSPHERIC PRESSURE, AND SUBSTANTIALLY CONTINUOUSLY AGITATING THE INOCULATED CULTURE MEDIUM BY SWIRLING IT ABOUT IN A CONTAINER WHILE CIRCULATING IN CONTACT THEREWITH AN ATMOSPHERE CONTINUOUSLY MAINTAINED AT APPROXIMATELY 5% CO2 IN AIR.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1964-06-26 | 1964-12-26 | 1965-06-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1968-06-26 | 1968-12-26 | 1969-06-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1972-06-26 | 1972-12-26 | 1973-06-27 | $7700.00 | Window Closed / Lapsed |