Patent US3269918A successfully refreshed from Google Patents.
US3269918A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of purified glucose oxidase
Assignee: Bayer Corp
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Filing: 1964-04-20
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Granted: 1966-08-30
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Expires: 1984-04-20
Abstract
No abstract record available.
Inventors
- 👤 Barton Richard Russell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 02:58
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; B; B01; B01J; B01J41/00; B01J41/04; B01J41/07; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/816; Y10S435/8215; Y10S435/911; Y10S435/913; Y10S435/917
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
201 words
A PROCESS FOR THE PRODUCTION OF PURIFIED GLUCOSE OXIDASE WHICH CONSISTS ESSENTIALLY OF MIXING AQUEOUS MYCELIUM MATERIAL CONTAINING GLUCOSE OXIDASE AND IMPURITIES INCLUDING CATALASE WITH AMMONIUM SULFATE TO PRECIPITATE THE GLUCOSE OXIDASE, SAID AMMONIUM SULFATE BEING ADDED TO THE AQUEOUS MYCELIUM MATERIAL IN AN AMOUNT OF AT LEAST ABOUT 85 PERCENT OF ASTURATION, SEPARATING THE GLUCOSE OXIDASE PRECIPITATE FROM THE REMAINDER OF THE AQUEOUS MYCELIUM MATERIAL, DISSOLVING THE GLUCOSE OXIDASE PRECIPITIATE IN WATER, ADJUSTING THE PH OF A WEAK ANIONIC IONEXCHANGE MATERIAL TO ABOUT 4.5, CONTACTING THE AQUEOUS GLUCOSE OXIDASE SOLUTION WITH SAID WEAK ANIONIC ION-EXCHANGE MATERIAL WHEREIN THE GLUCOSE OXIDASE AND IMPURITIES ARE ABSORBED BY THE WEAK ANIONIC ION EXCHANGE MATERIAL TERIAL, WASHING THE WEAK ANIONIC ION-EXCHANGE MATERIAL CONTAINING THE GLUCOSE OXIDASE AND IMPURITIES WITH A BUFFER HAVING A MOLAR STRENGTH OF ABOUT 0.07 TO ABOUT 0.09 AND A PH OF ABOUT 4.3 TO ABOUT 4.7 TO REMOVE ADSORBED CATALASE AND OTHER IMPURITIES FROM THE WEAK ANIONIC ION-EXCHANGE MATERIAL AND THEN WASHING THE WEAK ANIONIC ION-EXCHANGE MATERIAL WITH A BUFFER HAVING A MOLAR STRENGTH OF ABOUT 0.095 TO ABOUT 0.11 AND A PH OF ABOUT 3.5 TO ABOUT 3.8 TO REMOVE PURIFIED GLUCOSE OXIDASE FROM THE WEAK ANIONIC ION-EXCHANGE MATERIAL.
Claim 2
Dependent Claim
201 words
A PROCESS FOR THE PRODUCTION OF PURIFIED GLUCOSE OXIDASE WHICH CONSISTS ESSENTIALLY OF MIXING AQUEOUS MYCELIUM MATERIAL CONTAINING GLUCOSE OXIDASE AND IMPURITIES INCLUDING CATALASE WITH AMMONIUM SULFATE TO PRECIPITATE THE GLUCOSE OXIDASE, SAID AMMONIUM SULFATE BEING ADDED TO THE AQUEOUS MYCELIUM MATERIAL IN AN AMOUNT OF AT LEAST ABOUT 85 PERCENT OF ASTURATION, SEPARATING THE GLUCOSE OXIDASE PRECIPITATE FROM THE REMAINDER OF THE AQUEOUS MYCELIUM MATERIAL, DISSOLVING THE GLUCOSE OXIDASE PRECIPITIATE IN WATER, ADJUSTING THE PH OF A WEAK ANIONIC IONEXCHANGE MATERIAL TO ABOUT 4.5, CONTACTING THE AQUEOUS GLUCOSE OXIDASE SOLUTION WITH SAID WEAK ANIONIC ION-EXCHANGE MATERIAL WHEREIN THE GLUCOSE OXIDASE AND IMPURITIES ARE ABSORBED BY THE WEAK ANIONIC ION EXCHANGE MATERIAL TERIAL, WASHING THE WEAK ANIONIC ION-EXCHANGE MATERIAL CONTAINING THE GLUCOSE OXIDASE AND IMPURITIES WITH A BUFFER HAVING A MOLAR STRENGTH OF ABOUT 0.07 TO ABOUT 0.09 AND A PH OF ABOUT 4.3 TO ABOUT 4.7 TO REMOVE ADSORBED CATALASE AND OTHER IMPURITIES FROM THE WEAK ANIONIC ION-EXCHANGE MATERIAL AND THEN WASHING THE WEAK ANIONIC ION-EXCHANGE MATERIAL WITH A BUFFER HAVING A MOLAR STRENGTH OF ABOUT 0.095 TO ABOUT 0.11 AND A PH OF ABOUT 3.5 TO ABOUT 3.8 TO REMOVE PURIFIED GLUCOSE OXIDASE FROM THE WEAK ANIONIC ION-EXCHANGE MATERIAL.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1969-08-29 | 1970-02-28 | 1970-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1973-08-29 | 1974-02-28 | 1974-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1977-08-29 | 1978-02-28 | 1978-08-30 | $7700.00 | Window Closed / Lapsed |