Patent US3255080A successfully refreshed from Google Patents.
US3255080A
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Live rabies virus vaccine and method for the production thereof
Assignee: Dow Chemical Co
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Filing: 1962-08-06
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Granted: 1966-06-07
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Expires: 1982-08-06
Abstract
No abstract record available.
Inventors
- 👤 Jerrell B Emery
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 08:24
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/205; A61K2039/51; A61K2039/525; A61K2039/5254; A61K2039/55; A61K2039/552; C; C12; C12N; C12N2760/00; C12N2760/00011; C12N2760/20011; C12N2760/20111; C12N2760/20134
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
137 words
A METHOD FOR PROPAGATING A NODIFIED LIVE RABIES VIRUS WHICH COMPRISES THE STEPS OF CULTURING CHICK EMBRYO CELLS IN VITRO N A MEDIUM CAPABLE OF FOSTERING GROWTH OF SUCH CELLS TO PRODUCE MONOLAYER TISSUE CULTURES, DECANTING THE GROWTH MEDIUM FROM THE CULTURED CELLS, WASHING SAID CELLS WITH A PROTEIN-FREE NUTRIENT MEDIUM, INOCULATING THE CULTURED CELLS WITH AN AQUEOUS SUSPENSION OF LIVE RABIES VIRUS OF THE FLURY STRAIN WHICH HAS BEEN FURTHER MODIFIED BY AT LEAST ABOUT 64 SUCCESSIVE PASSAGES IN EMBRYONATED EGGS, ADDING TO THE CULTURED CELLS A SYNTHETIC MAINTENANCE MEDIUM SELECTED FROM THE GROUP CONSISTING OF MEDIUM 199, MEDIUM 858 AND EAGLE''S MEDIUM AND MAINTAINING THE RESULTING CULTURE AT A TEMPERATURE OF FROM ABOUT 34*C. TO 37*C. FOR A PERIOD OF FROM ABOUT 4 TO 14 DAYS TO ACCOMPLISH MULTIPLICATION OF THE VIRUS IN SAID CELLS.
Claim 2
Independent Claim
137 words
A METHOD FOR PROPAGATING A NODIFIED LIVE RABIES VIRUS WHICH COMPRISES THE STEPS OF CULTURING CHICK EMBRYO CELLS IN VITRO N A MEDIUM CAPABLE OF FOSTERING GROWTH OF SUCH CELLS TO PRODUCE MONOLAYER TISSUE CULTURES, DECANTING THE GROWTH MEDIUM FROM THE CULTURED CELLS, WASHING SAID CELLS WITH A PROTEIN-FREE NUTRIENT MEDIUM, INOCULATING THE CULTURED CELLS WITH AN AQUEOUS SUSPENSION OF LIVE RABIES VIRUS OF THE FLURY STRAIN WHICH HAS BEEN FURTHER MODIFIED BY AT LEAST ABOUT 64 SUCCESSIVE PASSAGES IN EMBRYONATED EGGS, ADDING TO THE CULTURED CELLS A SYNTHETIC MAINTENANCE MEDIUM SELECTED FROM THE GROUP CONSISTING OF MEDIUM 199, MEDIUM 858 AND EAGLE''S MEDIUM AND MAINTAINING THE RESULTING CULTURE AT A TEMPERATURE OF FROM ABOUT 34*C. TO 37*C. FOR A PERIOD OF FROM ABOUT 4 TO 14 DAYS TO ACCOMPLISH MULTIPLICATION OF THE VIRUS IN SAID CELLS.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1969-06-06 | 1969-12-06 | 1970-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1973-06-06 | 1973-12-06 | 1974-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1977-06-06 | 1977-12-06 | 1978-06-07 | $7700.00 | Window Closed / Lapsed |