Patent US3220929A successfully refreshed from Google Patents.
US3220929A
Expired / Lapsed
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Microbiological production of amino acid by reductive amination
Assignee: KH Neochem Co Ltd
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Filing: 1964-02-10
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Granted: 1965-11-30
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Expires: 1984-02-10
Abstract
No abstract record available.
Inventors
- 👤 Kinoshita Shukuo
- 👤 Tanaka Katsunobu
- 👤 Udaka Shigezo
- 👤 Akita Sadao
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:31
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/14; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
164 words
A FERMENTATIVE METHOD OF PRODUCING L-GLUTAMIC ACID BY REDUCTIVE AMINATION WHICH COMPRISES THE STEPS OF (1) CULTURING A MCROORGANISM IN A LIQUID MEDIUM CONTAINING AS ESSENTIAL CONSTITUENTS A SACCHARINE MATERIAL AND A NITROGEN SOURCE, WHICH MICROORGANISM POSSESSES; (A) THE ABILITY TO PRODUCE A-KETOGLUTARIC ACID FROM SACCHARINE MATERIAL, AND (B) STRONG L-GLUTAMIC ACID DEHYDROGENASE ACTIVITY, AND IN WHICH MICROORGANISM THE ENZYME EFFICIENCY VALUE IS AT LEAST 10%, THIS VALUE BEING OBTAINED BY CORRELATING THE CAPACITY OF THE DEHYDROGENASE ENZYME TO PRODUCE GLUTAMIC ACID IN THE REVERSIBLE ENZYMATIC REACTION WITH THE CAPACITY OF THE MICROORGANISM TO PRODUCE GLUTAMIC ACID BY FERMENTATION FROM SACCHARINE MATERIAL IN THE PRESENCE OF A NITROGEN SOURCE; (2) MAINTAINING THE PH OF THE CULTURING MEDIUM WITHIN THE RANGE OF FROM 6 TO 9 BY ADDITION OF NEUTRALIZING AGENT UNTIL A SUBSTANTIAL AMOUNT OF A PRODUCT SELECTED FROM THE GROUP CONSISTING OF L-GLUTAMIC ACID AND A SALT THEREOF IS ACCUMULATED IN THE MEDIUM; AND (3) RECOVERING THE THUS FORMED PRODUCT FROM SAID MEDIUM.
Claim 2
Dependent Claim
164 words
A FERMENTATIVE METHOD OF PRODUCING L-GLUTAMIC ACID BY REDUCTIVE AMINATION WHICH COMPRISES THE STEPS OF (1) CULTURING A MCROORGANISM IN A LIQUID MEDIUM CONTAINING AS ESSENTIAL CONSTITUENTS A SACCHARINE MATERIAL AND A NITROGEN SOURCE, WHICH MICROORGANISM POSSESSES; (A) THE ABILITY TO PRODUCE A-KETOGLUTARIC ACID FROM SACCHARINE MATERIAL, AND (B) STRONG L-GLUTAMIC ACID DEHYDROGENASE ACTIVITY, AND IN WHICH MICROORGANISM THE ENZYME EFFICIENCY VALUE IS AT LEAST 10%, THIS VALUE BEING OBTAINED BY CORRELATING THE CAPACITY OF THE DEHYDROGENASE ENZYME TO PRODUCE GLUTAMIC ACID IN THE REVERSIBLE ENZYMATIC REACTION WITH THE CAPACITY OF THE MICROORGANISM TO PRODUCE GLUTAMIC ACID BY FERMENTATION FROM SACCHARINE MATERIAL IN THE PRESENCE OF A NITROGEN SOURCE; (2) MAINTAINING THE PH OF THE CULTURING MEDIUM WITHIN THE RANGE OF FROM 6 TO 9 BY ADDITION OF NEUTRALIZING AGENT UNTIL A SUBSTANTIAL AMOUNT OF A PRODUCT SELECTED FROM THE GROUP CONSISTING OF L-GLUTAMIC ACID AND A SALT THEREOF IS ACCUMULATED IN THE MEDIUM; AND (3) RECOVERING THE THUS FORMED PRODUCT FROM SAID MEDIUM.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1968-11-29 | 1969-05-31 | 1969-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1972-11-29 | 1973-05-31 | 1973-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1976-11-29 | 1977-05-31 | 1977-11-30 | $7700.00 | Window Closed / Lapsed |