Patent US3127327A successfully refreshed from Google Patents.
US3127327A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Extraction of protease from a culture
Assignee: —
•
Filing: —
•
Granted: 1964-03-31
•
Expires: 1981-03-31
Abstract
No abstract record available.
Inventors
No inventors recorded.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 03:17
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/8215; Y10S435/822; Y10S435/886; Y10S435/897
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
90 words
A PROTEASE PRODUCED IN AND ISOLATED FROM THE CULTURE BROTH OF STREPTOMYCES GRISEUS AND POSSESSING THE FOLLOWING PROPERTIES: MOLECULAR WEIGHT MSD=19,20020,800; SEDIMENTATION CONSTANT S20,W=2.8S; DIFFUSION CONSTANT DA20,W=13.1 OR DU20,W=13.4X10**-7 (CM.2/SEC.); ELEMENTAL COMPOSITION C, 52.0%; H, 6.8%; N, 14.8%; CA, 0.8%; OPTIMUM PH FOR THE ENZYME ACTIVITY 7.5-8.5; HYDROLYZING ACTIVITY FOR PROTEIN: EXTREMELY STRONG; SAID PROTEASE HAVING BOTH "ENDO-PEPTIDASE" AND "EXO-PEPTIDASE" ENZYMATIC ACTION; SUBSTRATE SPECIFICITY; EXTREMELY BROAD; FIRST ORDER PROTEOLYTIC COEFFICIENT C=3.34X10**-2 FOR GLYCYL-L-LEUCINE, C=0.18X10**-2 FOR CARBOBENZOXY-LGLUTAMYL-L-TYROSINE; MICHAELIS'' CONSTANT FOR GLYCYL-L-LEU CINE KM=1.25X10**-2 M; ENERGY OF ACTIVATION FOR GLYCYLL-LEUCINE EA=2.7X10**3 CAL./MOL.
Claim 2
Dependent Claim
90 words
A PROTEASE PRODUCED IN AND ISOLATED FROM THE CULTURE BROTH OF STREPTOMYCES GRISEUS AND POSSESSING THE FOLLOWING PROPERTIES: MOLECULAR WEIGHT MSD=19,20020,800; SEDIMENTATION CONSTANT S20,W=2.8S; DIFFUSION CONSTANT DA20,W=13.1 OR DU20,W=13.4X10**-7 (CM.2/SEC.); ELEMENTAL COMPOSITION C, 52.0%; H, 6.8%; N, 14.8%; CA, 0.8%; OPTIMUM PH FOR THE ENZYME ACTIVITY 7.5-8.5; HYDROLYZING ACTIVITY FOR PROTEIN: EXTREMELY STRONG; SAID PROTEASE HAVING BOTH "ENDO-PEPTIDASE" AND "EXO-PEPTIDASE" ENZYMATIC ACTION; SUBSTRATE SPECIFICITY; EXTREMELY BROAD; FIRST ORDER PROTEOLYTIC COEFFICIENT C=3.34X10**-2 FOR GLYCYL-L-LEUCINE, C=0.18X10**-2 FOR CARBOBENZOXY-LGLUTAMYL-L-TYROSINE; MICHAELIS'' CONSTANT FOR GLYCYL-L-LEU CINE KM=1.25X10**-2 M; ENERGY OF ACTIVATION FOR GLYCYLL-LEUCINE EA=2.7X10**3 CAL./MOL.
Claim 3
Independent Claim
37 words
A METHOD COMPRISING CULTURING STREPTOMYCES GRISEUS TO FORM A CULTURE BROTH CONTAINING STREPTOMYCIN AND PROTEASE AND SEPARATELY ISOLATING THE STREPTOMYCIN AND THE PROTEASE, THE LATTER HAVING AN EXTREMELY STRONG HYDROLYZING ACTIVITY FOR PROTEIN BOTH "ENDO-PEPTIDASE" AND "EXOPEPTIDASE."
Claim 4
Independent Claim
37 words
A METHOD COMPRISING CULTURING STREPTOMYCES GRISEUS TO FORM A CULTURE BROTH CONTAINING STREPTOMYCIN AND PROTEASE AND SEPARATELY ISOLATING THE STREPTOMYCIN AND THE PROTEASE, THE LATTER HAVING AN EXTREMELY STRONG HYDROLYZING ACTIVITY FOR PROTEIN BOTH "ENDO-PEPTIDASE" AND "EXOPEPTIDASE."
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1967-03-31 | 1967-09-30 | 1968-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1971-03-31 | 1971-09-30 | 1972-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1975-03-31 | 1975-09-30 | 1976-03-31 | $7700.00 | Window Closed / Lapsed |