Patent US3098015A successfully refreshed from Google Patents.
US3098015A
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Enzyme purification
Assignee: Phadia AB
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Filing: 1962-01-08
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Granted: 1963-07-16
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Expires: 1982-01-08
Abstract
No abstract record available.
Inventors
- 👤 Ayrapaa Teuvo Pekka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:52
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/315; C07K14/3153; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
131 words
THE METHOD FOR PURIFYING ENZYMES SELECTED FROM THE GROUP CONSISTING OF STREPTOKINASE, STREPTODORNASE, AND MIXTURES THEREOF RENDER THE SAME SUBSTANTIALLY FREE OF IMPURITIES SUCH AS PYROGENS, HEMOLYSINS AND HYALURONIDASE, THAT COMPRISES TREATING A STARTING MATERIAL, SELECTED FROM THE GROUP CONSISTING OF FILTERED MASH PRODUCED BY FERMENTATION OF STREPTOCOCCI IN SUITABLE NUTRIENT MEDIUM AND ENZYME CONCENTRATES PREPARED THEREFROM, AT A BUFFERED ALKALINE PH WITH A SURFACE ACTIVE AGENT SELECTED FROM THE GROUP CONSISTING OF ANIONIC, CATIONIC AND NONIONIC SURFACE ACTIVE AGENTS HAVING THE CAPACITY TO FORM ASSOCIATION COLLOIDS IN WATER SOLUTION, WHEREBY AN INTERACTION BETWEEN SAID SURFACE ACTIVE AGENT AND IMPURITIES IS ATTAINED INACTIVATING THE SAME WITHOUT SUBSTANTIAL IMPAIRMENT OF SAID ENZYMES, AND SEPARATING SAID ENZYMES FROM THE SURFACE ACTIVE AGENT PREPARATORY TO CONCENTRATION AND RECOVERY OF SAID ENZYMES BY KNOWN METHODS.
Claim 2
Independent Claim
131 words
THE METHOD FOR PURIFYING ENZYMES SELECTED FROM THE GROUP CONSISTING OF STREPTOKINASE, STREPTODORNASE, AND MIXTURES THEREOF RENDER THE SAME SUBSTANTIALLY FREE OF IMPURITIES SUCH AS PYROGENS, HEMOLYSINS AND HYALURONIDASE, THAT COMPRISES TREATING A STARTING MATERIAL, SELECTED FROM THE GROUP CONSISTING OF FILTERED MASH PRODUCED BY FERMENTATION OF STREPTOCOCCI IN SUITABLE NUTRIENT MEDIUM AND ENZYME CONCENTRATES PREPARED THEREFROM, AT A BUFFERED ALKALINE PH WITH A SURFACE ACTIVE AGENT SELECTED FROM THE GROUP CONSISTING OF ANIONIC, CATIONIC AND NONIONIC SURFACE ACTIVE AGENTS HAVING THE CAPACITY TO FORM ASSOCIATION COLLOIDS IN WATER SOLUTION, WHEREBY AN INTERACTION BETWEEN SAID SURFACE ACTIVE AGENT AND IMPURITIES IS ATTAINED INACTIVATING THE SAME WITHOUT SUBSTANTIAL IMPAIRMENT OF SAID ENZYMES, AND SEPARATING SAID ENZYMES FROM THE SURFACE ACTIVE AGENT PREPARATORY TO CONCENTRATION AND RECOVERY OF SAID ENZYMES BY KNOWN METHODS.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1966-07-15 | 1967-01-14 | 1967-07-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1970-07-15 | 1971-01-14 | 1971-07-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1974-07-15 | 1975-01-14 | 1975-07-16 | $7700.00 | Window Closed / Lapsed |