Method for the production of carotenes

Claim 1

A METHOD FOR PRODUCING BETA-CAROTENE COMPRISING INCUBATING AN ORGANISM SELECTED FROM THE GROUP CONSISTING OF AN MATED STRAIN AND MATED (+) AND (-) STRAINS OF BETA-CAROTENE-PRODUCING ORGANISMS SELECTED FROM THE SPECIES CONSISTING OF BLAKESLEA TRISPORA AND CHOUNEPHORA CONJUNCTA IN AN AQUEOUS NUTRIENT MEDIUM HAVING A PH RANGING ABOUT FROM 5.5 TO 7.0 AND COMPOSED OF AN ASSIMILABLE CARBON SOURCE, AN ASSIMILABLE NITROGEN SOURCE, A THIAMIN SOURCE NUTRIENT MINERAL SALTS, AN ASSIMIBLE LIPID MATERIAL SELECTED FROM THE GROUP CONSISTING OF A NATURALLY OCCURING FAT AND OIL AND THE FATTY ACID COMPONENTS THEREOF, A SURFACE ACTIVE AGENT SELECTED FROM THE GROUP CONSISTING OF AN ALKYL ARYL POLYETHER ALCOHOL AND THE REACTION PRODUCT OF A FATTY ACID AND SORBITOL, AND BETA-IONONE, SAID LIPID MATERIAL BEING EMPLOYED AT A LEVEL OF ABOUT FROM 1 TO 6 PERCENT OF THE NUTRIENT MEDIUM, SAID SURFACE ACTIVE AGENT BEING EMPLOYED AT A LEVEL OF ABOUT FROM 0.0K TO 1 PERCENTOF THE NUTRIENT MEDIUM, AND SAID BETA-IONONE BEING EMPLOYED AT A LEVEL OF ABOUT FROM 10 TO 500 MICROGRAMS PER 100 MILLILITERS OF THE NUTRIENT MEDIUM, SAID INCUBATION BEING CARRIED OUT UNDER SUBMERGED AEROBIC CONDITIONS AT A TEMPERATURE RANGING FROM ABOUT 20* TO 350* C FOR A PERIOD OF ABOUT FROM 2 TO 7 DAYS TO PRODUCE BETA-CAROTENE.

Claim 2

A METHOD FOR PRODUCING BETA-CAROTENE COMPRISING INCUBATING AN ORGANISM SELECTED FROM THE GROUP CONSISTING OF AN MATED STRAIN AND MATED (+) AND (-) STRAINS OF BETA-CAROTENE-PRODUCING ORGANISMS SELECTED FROM THE SPECIES CONSISTING OF BLAKESLEA TRISPORA AND CHOUNEPHORA CONJUNCTA IN AN AQUEOUS NUTRIENT MEDIUM HAVING A PH RANGING ABOUT FROM 5.5 TO 7.0 AND COMPOSED OF AN ASSIMILABLE CARBON SOURCE, AN ASSIMILABLE NITROGEN SOURCE, A THIAMIN SOURCE NUTRIENT MINERAL SALTS, AN ASSIMIBLE LIPID MATERIAL SELECTED FROM THE GROUP CONSISTING OF A NATURALLY OCCURING FAT AND OIL AND THE FATTY ACID COMPONENTS THEREOF, A SURFACE ACTIVE AGENT SELECTED FROM THE GROUP CONSISTING OF AN ALKYL ARYL POLYETHER ALCOHOL AND THE REACTION PRODUCT OF A FATTY ACID AND SORBITOL, AND BETA-IONONE, SAID LIPID MATERIAL BEING EMPLOYED AT A LEVEL OF ABOUT FROM 1 TO 6 PERCENT OF THE NUTRIENT MEDIUM, SAID SURFACE ACTIVE AGENT BEING EMPLOYED AT A LEVEL OF ABOUT FROM 0.0K TO 1 PERCENTOF THE NUTRIENT MEDIUM, AND SAID BETA-IONONE BEING EMPLOYED AT A LEVEL OF ABOUT FROM 10 TO 500 MICROGRAMS PER 100 MILLILITERS OF THE NUTRIENT MEDIUM, SAID INCUBATION BEING CARRIED OUT UNDER SUBMERGED AEROBIC CONDITIONS AT A TEMPERATURE RANGING FROM ABOUT 20* TO 350* C FOR A PERIOD OF ABOUT FROM 2 TO 7 DAYS TO PRODUCE BETA-CAROTENE.