Plasma fractionation and product therefrom

Claim 1

A PROCESS FOR THE PREPARATION OF A SUBSTANCE CAPABLE OF INCREASING THE ACTIVITY OF THROMBOPLASTIC MATERIAL, CONDUCTED AT LOW TEMPERATURE THROUGHOUTY, COMPRISING THE STEPS: ADDING TO A UNIT COLUME OF PROTHROMBIN-FREE PLASMA SELECTED FROM THE CLASS CONSISTING OF HORSE, BOVINE, HUMAN, AND RABBIT PROTHROMBIN-FREE PLASMA, THREE TO FIVE COLUMES OF A FIRST AQUEOUS ALCOHOL SOLUTIONA BUFFERED AT A PH OF 4-5 AND CONTAINING ABOUT 15-30 PERCENT BY VOLUME OF AN ALCOHOL SELECTED FROM THE CLASS CONSISTING OF METHANOL AND ETHANOL AND MIXTURES THEREOF, STIRRUNG AND CENTRIFUGUNG THE MIXTURE, SEPARATING THE SUPERNATANT FROM THE RESIDUE; AND ADDING TO THE RESIDUE THREE TO FIVE VOLUMES BASED ONB PLASMA VOLUME OF A SECOND AQUEOUS ALCOHOL SOLUTION BUFFERED AT A PH OF 6-8 AND CONTAINING 5-20 PRECENT BY VOLUME OF AN ALCOHOL SELECTED FROM THE CLASS CONSISTING OF METHANOL AND ETHANOL AND MIXTURES THEREOF AND 4-6 PERCENT BY WEIGHT OF AN ALKALI METAL SALT OF AN AMINO ACID HAVING NOT MORE THAN SIX CARBON ATOMS, STIRRING AND CETRIFUGING THE MIXTURE, FILTERING THE SUPERNATANT, DIALYZING THE FILTERED SUPERANATANT AGAINST DISTILLED WATER, AND LYOPHILIZING THE DIALYZED SUPERNATANT.