Medium and method for the mass culture of microorganisms

Claim 1

A PROCESS FOR THE MASS CULTURE OF A MICROORGANISM WHICH COMPRISES GROWING SAID MICROORGANISM IN A MEDIUM FOR SAID MICROORGANISM CONTAINING THOSE ELEMENTS NECESSARY IN TRACE AMOUNTS FOR THE GROWTH OF SAID MICROORGANISM, SAID ELEMENTS BEING PRESENT IN SAID MEDIUM IN AMOUNTS SUFFICIENT TO SUPPORT A HEAVY GROWTH OF SAID MICROORGANISM THEREIN, TOGETHER WITH A NON-METABOLIZABLE CHELATING AGENT SELECTED FROM A GROUP CONSISTING OF ETHYLENEDIAMINE TETRAACETIC ACID, THE TETRASODIUM SALT OF SAID ACID AND THE CALCIUM DISODIUM SALT OF SAID ACID, SAID CHELATING AGENT CAPABLE OF FORMING SOLUBLE, NON-METABOLIZABLE COMPLEXES WITH SAID ELEMENTS, SAID CHELATING AGENT BEING PRESENT IN SAID MEDIUM IN AN AMOUNT SUFFICIENT TO REDUCE THE EFFECTIVE CONCENTRATION OF THE TRACE ELEMENTS THEREIN BELOW THEIR MICROORGANISM GROWTH INHIBITORY VALUE AND A NON-METABOLIZABLE PH BUFFER FOR SAID MEDIUM SELECTED FROM A GROUP CONSISTING OF TRIETHANOLAMINE, DIHYDROXYETHYL ETHYLENEDIAMINE, TRIS-HYDROXYAMINO METHANE, 2-AMINO-2-METHYL PROPANE DIOL, TRANSACONITIC ACID AND TETRACARBOXYBUTANE, SAID BUFFER BEING IN AN AMOUNT SUFFICIENT TO MAINTAIN SAID MEDIUM DURING THE GROWTH OF SAID MICROORGANISM AT A PH IN THE RANGE SUITABLE FOR THE GROWTH OF SAID MICROORGANISM.