Patent US2720527A successfully refreshed from Google Patents.
US2720527A
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Synthesis of biocytin
Assignee: Merck and Co Inc
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Filing: 1951-10-12
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Granted: 1955-10-11
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Expires: 1971-10-12
Abstract
No abstract record available.
Inventors
- 👤 Donald E Wolf
- 👤 Folkers Karl
- 👤 Robert L Peck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 20:29
CPC Classifications
C; C07; C07D; C07D495/00; C07D495/02; C07D495/04
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
71 words
THE PROCESS THAT COMPRISES INTIMATELY CONTACTING AN ALKALINE AQUEOUS SOLUTION OF L-LYSINE COPPER COMPLEX WITH A SUSPENSION OF BIOTIN ACID HALIDE IN A WATER-IMMISCIBLE INERT ORGANIC SOLVENT, MAINTAINING PH OF THE REACTION MIXTURE AT APPROXIMATELY PH 8, AND THE TEMPERATURE AT BETWEEN -15* C. AND ROOM TEMPERATURE TO FORM E-N-(DBIOTINYL)-L-LYSINE COPPER COMPLEX, ACIDIFYING THE LATTER COMPOUND WITH AN INORGANIC MINERAL ACID, REMOVING THE COPPER AS AN INSOLUBLE SALT AND RECOVERING E-N-(DBIOTINYL)-L-LYSINE.
Claim 2
Dependent Claim
71 words
THE PROCESS THAT COMPRISES INTIMATELY CONTACTING AN ALKALINE AQUEOUS SOLUTION OF L-LYSINE COPPER COMPLEX WITH A SUSPENSION OF BIOTIN ACID HALIDE IN A WATER-IMMISCIBLE INERT ORGANIC SOLVENT, MAINTAINING PH OF THE REACTION MIXTURE AT APPROXIMATELY PH 8, AND THE TEMPERATURE AT BETWEEN -15* C. AND ROOM TEMPERATURE TO FORM E-N-(DBIOTINYL)-L-LYSINE COPPER COMPLEX, ACIDIFYING THE LATTER COMPOUND WITH AN INORGANIC MINERAL ACID, REMOVING THE COPPER AS AN INSOLUBLE SALT AND RECOVERING E-N-(DBIOTINYL)-L-LYSINE.
References Cited (Backward Citations)
US2462124A
Cited
US2519530A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1958-10-10 | 1959-04-11 | 1959-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1962-10-10 | 1963-04-11 | 1963-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1966-10-10 | 1967-04-11 | 1967-10-11 | $7700.00 | Window Closed / Lapsed |