Patent US2571126A successfully refreshed from Google Patents.
US2571126A
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Hot aqueous extraction of enzymes from insulin-free pancreas
Assignee: Novo Terapeutisk Laboratorium AS
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Filing: 1946-10-23
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Granted: 1951-10-16
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Expires: 1966-10-23
Abstract
No abstract record available.
Inventors
- 👤 Frederiksen Svend Emil
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 14:14
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/94; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
128 words
A METHOD OF RECOVERING INSULIN AND PROTEOLYTIC ENZYMES FROM MAMMALIAN PANCREAS GLANDS, WHICH COMPRISES TREATING A MINCED PANCREAS GLAND MATERIAL WITH A CONVENTIONAL INSULIN EXTRACTING CONCENTRATION OF A SOLVENT SELECTED FROM THE GROUP CONSISTING OF ALIPHATIC ALCOHOLS CONTAINING LESS THAN THREE CARBON ATOMS AND ACETONE ACIDIFIED WITH SULPHURIC ACID TO CREATE A PH-VALUE BELOW 7 DURING THE SAID TREATMENT, THEREBY BRINGING THE INSULIN INTO SOLUTION AND PRECIPITATING THE ENZYMES, SEPARATNG THE INSULINCONTAINING LIQUID FROM THE TREATED GLAND MATERIAL, RECOVERING THE DISSOLVED INSULIN FROM THE LIQUID, ADDING WATER TO THE RESIDUAL GLAND MATERIAL, ACIDIFYING THE AQUEOUS MIXTURE TO PH 1 TO 4, HEATING THE MIXTURE QUICKLY TO 100* C., PROMPTLY COOLING THE MIXTURE, SEPARATING THE LIQUID FROM THE RESIDUAL GLAND MATERIAL, AND RECOVERING THE ENZYMES FROM THE SEPARATED LIQUID.
Claim 2
Independent Claim
128 words
A METHOD OF RECOVERING INSULIN AND PROTEOLYTIC ENZYMES FROM MAMMALIAN PANCREAS GLANDS, WHICH COMPRISES TREATING A MINCED PANCREAS GLAND MATERIAL WITH A CONVENTIONAL INSULIN EXTRACTING CONCENTRATION OF A SOLVENT SELECTED FROM THE GROUP CONSISTING OF ALIPHATIC ALCOHOLS CONTAINING LESS THAN THREE CARBON ATOMS AND ACETONE ACIDIFIED WITH SULPHURIC ACID TO CREATE A PH-VALUE BELOW 7 DURING THE SAID TREATMENT, THEREBY BRINGING THE INSULIN INTO SOLUTION AND PRECIPITATING THE ENZYMES, SEPARATNG THE INSULINCONTAINING LIQUID FROM THE TREATED GLAND MATERIAL, RECOVERING THE DISSOLVED INSULIN FROM THE LIQUID, ADDING WATER TO THE RESIDUAL GLAND MATERIAL, ACIDIFYING THE AQUEOUS MIXTURE TO PH 1 TO 4, HEATING THE MIXTURE QUICKLY TO 100* C., PROMPTLY COOLING THE MIXTURE, SEPARATING THE LIQUID FROM THE RESIDUAL GLAND MATERIAL, AND RECOVERING THE ENZYMES FROM THE SEPARATED LIQUID.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1954-10-15 | 1955-04-16 | 1955-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1958-10-15 | 1959-04-16 | 1959-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1962-10-15 | 1963-04-16 | 1963-10-16 | $7700.00 | Window Closed / Lapsed |