US20040137460A1
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US
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Genes with es cell-specific expression
Assignee: Sumitomo Pharma Co Ltd
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Filing: 2002-05-31
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Granted: —
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Expires: 2022-05-31
Abstract
Abstract The present invention relates to a probe for selecting ES cells, which characteristically contains one of DNAs having base sequences depicted in SEQ ID Nos; 1, 2, 3, 4, 5, 6, 7 and 8, or DNAs having base sequences depicted in SEQ ID Nos; 9, 11, 13, 15, 17, 19, 21, 23 and 41 and a screening method of ES cell using this probe. Preparation of a probe for selecting ES cells becomes feasible by identifying plural gene with ES cell-specific expressions (ECAT genes) and using the information of the base sequences of these gene groups. Efficient selection of ES cell enables supply of a large amount of ES cell expected to be applicable to regenerative medicine.
Inventors
- 👤 Shinya Yamanaka
- 👤 Eiko Kaiho
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 20:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6881; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0606; C12Q2600/00; C12Q2600/158
Claims Analysis
Total Claims: 58
Claim 1
Independent Claim
31 words
1 . A probe for selecting ES cells, comprising a DNA having a base sequence depicted in any one of SEQ ID Nos; 1, 3, 4, 5, 6, 7 and 8.
Claim 2
Dependent Claim
31 words
1 . A probe for selecting ES cells, comprising a DNA having a base sequence depicted in any one of SEQ ID Nos; 1, 3, 4, 5, 6, 7 and 8.
Claim 3
Dependent Claim
50 words
2 . A probe for selecting ES cells, comprising a DNA which hybridizes to a DNA having a base sequence depicted in any one of SEQ ID Nos; 1, 3, 4, 5, 6, 7 and 8 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 4
Dependent Claim
50 words
2 . A probe for selecting ES cells, comprising a DNA which hybridizes to a DNA having a base sequence depicted in any one of SEQ ID Nos; 1, 3, 4, 5, 6, 7 and 8 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 5
Dependent Claim
60 words
3 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in SEQ ID No; 1, 3, 4, 5, 6, 7 or 8, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 6
Dependent Claim
60 words
3 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in SEQ ID No; 1, 3, 4, 5, 6, 7 or 8, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 7
Dependent Claim
33 words
4 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in any one of SEQ ID Nos; 9, 13, 15, 17, 19, 21, 23 and 41.
Claim 8
Dependent Claim
33 words
4 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in any one of SEQ ID Nos; 9, 13, 15, 17, 19, 21, 23 and 41.
Claim 9
Dependent Claim
32 words
5 . The probe of claim 4 , comprising a DNA which has a base sequence depicted in any one of SEQ ID Nos; 9, 13, 15, 17, 19, 21 and 23.
Claim 10
Dependent Claim
51 words
6 . A probe for selecting ES cells, comprising a DNA which hybridizes to a DNA having a base sequence depicted in any one of SEQ ID Nos; 9, 13, 15, 17, 19, 21, 23 and 41 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 11
Dependent Claim
51 words
6 . A probe for selecting ES cells, comprising a DNA which hybridizes to a DNA having a base sequence depicted in any one of SEQ ID Nos; 9, 13, 15, 17, 19, 21, 23 and 41 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 12
Dependent Claim
51 words
7 . The probe of claim 6 , comprising a DNA which hybridizes to a DNA which has a base sequence depicted in any one of SEQ ID Nos; 9, 13, 15, 17, 19, 21 and 23 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 13
Dependent Claim
61 words
8 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in SEQ ID No; 9, 13, 15, 17, 19, 21, 23 or 41, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 14
Dependent Claim
61 words
8 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in SEQ ID No; 9, 13, 15, 17, 19, 21, 23 or 41, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 15
Dependent Claim
60 words
9 . The probe of claim 8 , comprising a DNA which has a base sequence depicted in SEQ ID No; 9, 13, 15, 17, 19, 21 or 23, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 16
Dependent Claim
21 words
10 . The probe of any of claims 1 to 9 , which is used for selecting a mouse ES cell.
Claim 17
Dependent Claim
32 words
11 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in any one of SEQ ID Nos; 27, 29, 31, 33, 35, 37 and 43.
Claim 18
Dependent Claim
32 words
11 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in any one of SEQ ID Nos; 27, 29, 31, 33, 35, 37 and 43.
Claim 19
Dependent Claim
32 words
12 . The probe of claim 11 , which comprises a DNA which has q base sequence depicted in any one of SEQ ID Nos.; 27, 29, 31, 33, 35 and 37.
Claim 20
Dependent Claim
51 words
13 . A probe for selecting ES cells, comprising a DNA which hybridizes to a DNA which has a base sequence depicted in any one of SEQ ID Nos; 27, 29, 31, 33, 35, 37 and 43 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 21
Dependent Claim
51 words
13 . A probe for selecting ES cells, comprising a DNA which hybridizes to a DNA which has a base sequence depicted in any one of SEQ ID Nos; 27, 29, 31, 33, 35, 37 and 43 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 22
Dependent Claim
51 words
14 . The probe of claim 13 , which comprises a DNA which hybridizes to a DNA which has a base sequence depicted in any one of SEQ ID Nos; 27, 29, 31, 33, 35 and 37 under stringent conditions, and which encodes a protein specifically expressed in an ES cell.
Claim 23
Dependent Claim
60 words
15 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in SEQ ID No; 27, 29, 31, 33, 35, 37 or 43, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 24
Dependent Claim
60 words
15 . A probe for selecting ES cells, comprising a DNA which has a base sequence depicted in SEQ ID No; 27, 29, 31, 33, 35, 37 or 43, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 25
Dependent Claim
59 words
16 . The probe of claim 15 , comprising a DNA which has a base sequence depicted in SEQ ID No; 27, 29, 31, 33, 35 or 37, wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 26
Dependent Claim
21 words
17 . The probe of any of claims 11 to 16 , which is used for selecting a human ES cell.
Claim 27
Dependent Claim
94 words
18 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 17 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 28
Dependent Claim
94 words
18 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 17 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 29
Dependent Claim
53 words
19 . A protein-of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 18 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 30
Dependent Claim
53 words
19 . A protein-of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 18 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 31
Dependent Claim
94 words
20 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 29 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 32
Dependent Claim
94 words
20 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 29 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 33
Dependent Claim
54 words
21 . A protein of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 30 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 34
Dependent Claim
54 words
21 . A protein of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 30 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 35
Dependent Claim
95 words
22 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 33 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, and which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 36
Dependent Claim
95 words
22 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 33 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, and which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 37
Dependent Claim
54 words
23 . A protein of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 34 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 38
Dependent Claim
54 words
23 . A protein of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 34 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 39
Dependent Claim
95 words
24 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 37 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, and which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 40
Dependent Claim
95 words
24 . A gene comprising a DNA of any of the following (a)-(c): (a) a DNA comprising a base sequence depicted in SEQ ID No; 37 (b) a DNA which hybridizes to a DNA having a base sequence of (a) under stringent conditions, and which encodes a protein specifically expressed in an ES cell (c) a DNA which has a base sequence of (a), wherein one to several bases are deleted, substituted or added, and which is capable of hybridizing, under stringent conditions, to a DNA encoding a protein specifically expressed in an ES cell.
Claim 41
Dependent Claim
54 words
25 . A protein of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 38 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 42
Dependent Claim
54 words
25 . A protein of the following (a) or (b): (a) a protein having an amino acid sequence depicted in SEQ ID No; 38 (b) a protein which has an amino acid sequence of (a), wherein one to several bases are deleted, substituted or added, and which is specifically expressed in an ES cell.
Claim 43
Dependent Claim
59 words
26 . A method of screening an ES cell, which comprises analyzing an intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 9, 13, 15, 17, 19, 21, 23 or 41, or a protein having an amino acid sequence depicted in SEQ ID No; 10, 14, 16, 18, 20, 22, 24 or 42.
Claim 44
Dependent Claim
59 words
26 . A method of screening an ES cell, which comprises analyzing an intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 9, 13, 15, 17, 19, 21, 23 or 41, or a protein having an amino acid sequence depicted in SEQ ID No; 10, 14, 16, 18, 20, 22, 24 or 42.
Claim 45
Dependent Claim
56 words
27 . The method of claim 26 , wherein the intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 9, 13, 15, 17, 19, 21 or 23, or a protein having an amino acid sequence depicted in SEQ ID No; 10, 14, 16, 18, 20, 22 or 24 is analyzed.
Claim 46
Dependent Claim
45 words
28 . The method of claim 26 or 27 , which further comprises analyzing an intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 11, or a protein having an amino acid sequence depicted in SEQ ID No; 12.
Claim 47
Dependent Claim
47 words
29 . The method of any of claims 26 to 28 , which further comprises analyzing an intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 25, or a protein having an amino acid sequence depicted in SEQ ID No; 26.
Claim 48
Dependent Claim
57 words
30 . A method of screening an ES cell, which comprises analyzing an intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 27, 29, 31, 33, 35, 37 or 43, or a protein having an amino acid sequence depicted in SEQ ID No; 28, 30, 32, 34, 36, 38 or 44.
Claim 49
Dependent Claim
57 words
30 . A method of screening an ES cell, which comprises analyzing an intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 27, 29, 31, 33, 35, 37 or 43, or a protein having an amino acid sequence depicted in SEQ ID No; 28, 30, 32, 34, 36, 38 or 44.
Claim 50
Dependent Claim
54 words
31 . The method of claim 30 , wherein the intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 27, 29, 31, 33, 35 or 37, or a protein having an amino acid sequence depicted in SEQ ID No; 28, 30, 32, 34, 36 or 38 is analyzed.
Claim 51
Dependent Claim
45 words
32 . The method of claim 30 or 31 , which further comprises analyzing an intracellular expression state of a DNA having a base sequence depicted in SEQ ID No; 39, or a protein having an amino acid sequence depicted in SEQ ID No; 40.
Claim 52
Dependent Claim
69 words
33 . A probe for selecting ES cells, comprising a DNA which has a non-repetitive sequence comprising not less than 20 continuous bases from a base sequence depicted in SEQ ID No; 9, 11, 13, 15, 17, 19, 21, 23 or 41, or SEQ ID No; 27, 29, 31, 33, 35, 37 or 43, and which has a sequence specific to a gene specifically expressed in an ES cell.
Claim 53
Dependent Claim
69 words
33 . A probe for selecting ES cells, comprising a DNA which has a non-repetitive sequence comprising not less than 20 continuous bases from a base sequence depicted in SEQ ID No; 9, 11, 13, 15, 17, 19, 21, 23 or 41, or SEQ ID No; 27, 29, 31, 33, 35, 37 or 43, and which has a sequence specific to a gene specifically expressed in an ES cell.
Claim 54
Dependent Claim
37 words
34 . A method of screening an ES cell, which comprises analyzing an expression state of a gene specifically expressed in an ES cell, using a probe of any of claims 1 - 17 and 33 .
Claim 55
Dependent Claim
33 words
35 . The method of claim 34 , which further comprises using a probe for selecting ES cells comprising a DNA having a base sequence depicted in SEQ ID No; 2 or 11.
Claim 56
Dependent Claim
32 words
36 . The method of claim 34 or 35 , which further comprises using an ES cell selection probe comprising a DNA having a base sequence depicted in SEQ ID No; 25.
Claim 57
Dependent Claim
30 words
37 . The method of claim 34 , which further comprises using an ES cell selection probe comprising a DNA having a base sequence depicted in SEQ ID No; 27.
Claim 58
Dependent Claim
32 words
38 . The method of claim 34 or 35 , which further comprises using an ES cell selection probe comprising a DNA having a base sequence depicted in SEQ ID No; 39.
References Cited (Backward Citations)
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