Preparation of liposome containing sealed gene

Abstract

Abstract PURPOSE:To enable removal of microbial cells with a 0.45mum filter, by adding a polyhydric alcohol at a latter stage of a gene-inclusion process using a reverse- phase evaporation method, thereby efficiently including a DNA in a liposome having a diameter of <=450nm. CONSTITUTION:In the production of a gene-including liposome by a reverse- phase evaporation method, a polyhydric alcohol such as glycerol or ethylene glycol is added at the latter stage of the gene-inclusion process. The liposome suspension obtained by the above process is treated with a Nuclepore filter having an average pore size of 400nm to obtain liposomes composed mainly of those having diameter of 100-400nm. The gene to be included by this process may be a DNA or a recombinant DNA.

Inventors

• 👤 Kunio Yagi
• 👤 Nakao Kojima
• 👤 Nobuyuki Haga

Bibliographic Details