Biosensor

Abstract

Abstract PURPOSE:To obtain a biosensor having an excellent preservable property and high accuracy by providing a filter membrane, etc., between an oxidation- reduction enzyme layer and an electron acceptor layer. CONSTITUTION:The filter membrane 2, etc., are provided between the oxidation- reduction enzyme layer 10 and the electron acceptor layer 9. Whole blood is dropped as a sample liquid to the acceptor layer 9 and potassium ferricyanide is dissolved into the whole blood in the acceptor layer 9. Solid contents such as red blood cells are removed from the whole blood in which the potassium ferricyanide is dissolved at the time when said blood passes the filter membrane 2. The accuracy of measurement is degraded when the solid contents arrive at the electrode surface. An enzyme reaction takes place when the glucose oxidase of the enzyme layer 10 dissolved in the filtered sample liquid. Namely, the potassium ferricyanide is brought into reaction with glucose by the glucose oxidase and is changed to potassium ferrocyanide. The potassium ferrocyanide is electrochemically oxidized on a carbon electrode to form the potassium ferricyanide. This oxidation current corresponds to the concn. of the glucose which is the substrate.

Inventors

• 👤 Shigeo Kobayashi
• 👤 Shiro Nankai
• 👤 Kenichi Morigaki
• 👤 Mariko Kawaguri
• 👤 Sachiko Suetsugu
• 👤 Kiyomi Komatsu

Bibliographic Details