Cellulose gel having biochemical affinity and production thereof

Abstract

Abstract PURPOSE:To obtain the titled gel having epsilon-polylysine bonded through a functional group to a spherical cellulose particle, by reacting the spherical cellulose particle containing an introduced functional group to be reacted with epsilon-polylysine with the epsilon-polylysine. CONSTITUTION:A spherial cellulose particles to which a functional group (e.g. formly group, epoxy group, carboxyl group, etc.) to be reacted with epsilon-polylysine is reacted with the epsilon-polylysine and optionally subjected to aftertreatment to give the aimed gel. An epsilon-polylysine having 20-30 polymerization degree obtained by cultivating obtained by cultivating Streptomyces Albulus in a culture containing glycerol, ammonium sulfate, yeast essence, etc., followed by separation and purification is preferable as the epsilon-polylysine. Hard gel having high mechanical strength, useful for affinity chromatography showing high speed of flow and good purification efficiency free from release of ligand is obtained.

Inventors

• 👤 Hiroaki Ishibashi
• 👤 石橋　博明
• 👤 Shinichi Takasaki
• 👤 真一 高崎

Bibliographic Details