Novel csf and production thereof

Abstract

Abstract NEW MATERIAL:The CSF having the following physical and chemical properties. Molecular weight, 19,000+ or -1,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis; isoelectric point, PI=5.5+ or -0.1, 5.8+ or -0.1 or 6.1+ or -0.1; ultraviolet absorption, absorption peak at 280nm and minimum absorption at 250nm; amino acid sequence, 21 amino acids from N-terminal are shown in the formula. USE:Remedy and clinical examination reagent for hypoleukocytosis, etc. PREPARATION:The objective CSF can be produced e.g. by culturing a cell strain capable of producing human marrow cell differentiation and proliferation promoting factor (CSF) in a serum-free culture liquid, subjecting the supernatant liquid of culture product to gel-filtration, adsorbing the obtained fraction to reverse-phase high-performance liquid chromatography carrier and purifying the eluted fraction by high-performance molecular sieve chromatography and isoelectric electrophoresis, etc.

Inventors

• 👤 Masayoshi Ono
• 👤 尾野　雅義
• 👤 Hitoshi Nomura
• 👤 仁 野村

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