Method for quantifying prostate specific antigen and alpha1-antitrypsin composite

Abstract

Abstract PURPOSE:To make it possible to calculate the quantity of a gamma-Sm-a1-antitrypsin composite (gamma-Sm-a1AT) by using an anti-gamma-seminoprotein (gamma-Sm) antibody and an anti-a1-antitrypsin antibody. CONSTITUTION:The titled method can be performed by a sandwich immunoassay method. For example, anti-gamma-Sm antibody is adsorbed by or bonded to an insoluble carrier to form an insolubilized antibody. An anti-a1-antitrypsin antibody is labelled with a marker such as enzyme, an radioactive isotope or a fluorescent substance to obtain a labelled antibody. These antibodies are subjected to the antigen-antibody reaction with the gamma-Sm-a1AT composite in a specimen to be examined and the quantity of said composite can be calculated from the quantity of the bonded marker. Contrarily, the anti-a1-antitrypsin antibody may be converted to an insolubilized antibody while the anti-gamma-Sm antibody may be used as a marked antibody.

Inventors

• 👤 Shinichi Kamaike
• 👤 蒲池　信一
• 👤 Tomoko Maruyama
• 👤 智子 丸山

Bibliographic Details