Separation of nucleotide from nucleic acid base and nucleoside

Abstract

Abstract PURPOSE:To separate a high-quality nucleotide from nucleic acid base and nucleoside which are precursors of the nucleotide, in high efficinecy, by using a porous gel obtained by bonding a nucleic acid base to a porous gel containing epoxy group, treating the gel first with water, and then treating with ammonia water. CONSTITUTION:A nucleic acid base and its relating compound are treated first with water and then with ammonia water (preferably 10N-0.001N) using a porous gel contaning epoxy group and bonded with nucleic acid base, e.g. a porous gel containing nucleic acid base and obtained by reacting a cross-linked porous polyglycidyl methacrylate gel with a nucleic acid base in N,N-dimethylformamide. The nucleotide can be separated from the nucleic acid base and nucleoside by this process. USE:Nucleotide is useful as a raw material of synthetic DNA and RNA, or intermediate of pharmaceuticals. The nucleic acid base and nucleoside are precursors of nucleotide.

Inventors

• 👤 Toru Kiyota
• 👤 徹 清田

Bibliographic Details