Immobilization method for physiologically active substances

Abstract

Abstract PURPOSE:To immobilize the titled substance in a large amount to be immobilized with certainty, by dissolving a polyfunctional reagent in an acetic acid buffer solution having an adjusted molar concentration, pH value and ionic strength, reacting the polyfunctional reagent with chitosan in the above-mentioned solution, and adding the physiologically active substance thereto. CONSTITUTION:A normal salt, e.g. NaCl, is added to an acetic acid buffer solution within 0.05-0.20mol concentration range and a pH value within the range of the formula to adjust the ionic strength to 0.3-1.0. A polyfunctional reagent, e.g. glutaraldehyde, is then dissolved in the resultant solution and reacted with chitosan in the above-mentioned solution. A physiologically active substance, e.g. blood serum albumin, is then added thereto and immobilized on chitosan as a carrier.

Inventors

• 👤 Hiroaki Yabe
• 👤 谷邊　博昭
• 👤 Itsuo Kurahashi
• 👤 倉橋　五男
• 👤 Yoshihide Kawamura
• 👤 佳秀 川村
• 👤 Hiroshi Seo
• 👤 瀬尾　寛

Bibliographic Details