Patent EP0754466A2 successfully refreshed from Google Patents.
EP0754466A2
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EP
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Osteoinduction substance, method of manufacturing the same, and bone filling material including the same
Assignee: Matsumoto Dental College
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Filing: 1996-07-19
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Granted: —
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Expires: 2016-07-19
Abstract
Abstract An osteoinduction substance is extracted from animal bones and essentially comprises Mg, Si, Ca, and Na. The osteoinduction substance is manufactured by subjecting the animal bone to acid dipping, liming, neutralization, thermal extraction, condensation, and dehydration to obtain gelatin. The gelatin is then subjected to deproteinization under a nonheat or a low-temperature condition to extract the osteoinduction substance. The osteoinduction substance is mixed with at least one of hydroxyapatite powder and chitosan to form a bone filling material. When the osteoinduction substance is mixed with chitosan sol, a bone filling material of a sheet type is obtained.
Inventors
- 👤 Michio Ito
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 12:01
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3604; A61L27/3608; A61L27/02; A61L27/10; A61P; A61P1/00; A61P1/02; A61P43/00
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
50 words
An osteoinduction substance which contains Mg, Si, Ca, and Na and which is prepared by the steps of subjecting animal bones to liming, neutralization, thermal extraction, condensation, and dehydration to obtain gelatin; and subjecting said gelatin to deproteinization which is carried out through a nonheat process or a low-temperature process.
Claim 2
Dependent Claim
22 words
An osteoinduction substance as claimed in claim 1, wherein said deprotenization is carried out by dissolving said gelatin in alkaline aqueous solution.
Claim 3
Dependent Claim
19 words
An osteoinduction substance as claimed in claim 2, wherein NaOH aqueous solution is used as said alkaline aqueous solution.
Claim 4
Independent Claim
53 words
A method of manufacturing an osteoinduction substance, comprising the steps of subjecting animal bones to acid dipping, liming, neutralization, thermal extraction, condensation, and dehydration to obtain gelatin; subjecting said gelatin to deproteinization which is carried out through a nonheat process or a low-temperature process; and filtering said solution to extract said osteoinduction substance.
Claim 5
Dependent Claim
26 words
A method of manufacturing an osteoinduction substance as claimed in claim 4, wherein said deproteinization is carried out by dissolving said gelatin in alkaline aqueous solution.
Claim 6
Dependent Claim
23 words
A method of manufacturing an osteoinduction substance as claimed in claim 5, wherein NaOH aqueous solution is used as said alkaline aqueous solution.
Claim 7
Dependent Claim
35 words
A method of manufacturing an osteoinduction substance as claimed in claim 6, wherein said NaOH aqueous solution is heated during said deproteinization at a low temperature between 80°C and 85°C for two to three hours.
Claim 8
Dependent Claim
67 words
A bone filling material comprising a mixture of an osteoinduction substance and at least one of hydroxyapatite powder and chitosan, said osteoinduction substance containing Mg, Si, Ca, and Na and manufactured by the steps of subjecting animal bones to liming, neutralization, thermal extraction, condensation, and dehydration to obtain gelatin, and subjecting said gelatin to deproteinization which is carried out through a nonheat process or a low-temperature process.
Claim 9
Dependent Claim
23 words
A bone filling material as claimed in claim 8, wherein said deproteinization is carried out by dissolving said gelatin in alkaline aqueous solution.
Claim 10
Dependent Claim
20 words
A bone filling material as claimed in claim 9, wherein NaOH aqueous solution is used as said alkaline aqueous solution.
Claim 11
Dependent Claim
33 words
A bone filling material as claimed in claim 8 or 9, said material including chitosan sol which is gelatinized by said osteoinduction substance to form said bone filling material into a sheet type.
Claim 12
Dependent Claim
37 words
A material as claimed in any of claims 8 to 11, wherein said hydroxyapatite powder has a particle size between 10 and 50 microns and includes at least one of crystallized hydroxyapatite powder and amorphous hydroxyapatite powder.
Claim 13
Dependent Claim
21 words
A material as claimed in any of claims 8 to 12, wherein said hydroxyapatite powder is composed of amorphous hydroxyapatite powder.
Claim 14
Independent Claim
73 words
A method of manufacturing a bone filling material, comprising the steps of: preparing an osteoinduction substance by subjecting animal bones to acid dipping, liming, neutralization, thermal extraction, condensation, and dehydration to obtain gelatin, subjecting said gelatin to deproteinization which is carried out through a nonheat process or a low-temperature process, and filtering said solution to obtain said osteoinduction substance; and mixing said osteoinduction substance with at least one of hydroxyapatite powder and chitosan.
Claim 15
Dependent Claim
50 words
A method of manufacturing a bone filling material as claimed in claim 14, comprising the steps of dissolving hydroxyapatite powder and chitosan by acid using physiological saline solution to form a sol material; and kneading said osteoinduction substance into said sol material to form paste as said bone filling material.
Claim 16
Dependent Claim
43 words
A method of manufacturing a bone filling material as claimed in claim 15, said method further comprising the steps of dehydrating said paste by the use of liquid nitrogen, and shaping said paste into a sheet which serves as said bone filling material.
Claim 17
Dependent Claim
24 words
A method as claimed in claim 14, 15 or 16, wherein said deproteinization is carried out by dissolving said gelatin In alkaline aqueous solution.
Claim 18
Dependent Claim
24 words
A method of manufacturing a bone filling material as claimed in claim 17, wherein NaOH aqueous solution is used as said alkaline aqueous solution.
Claim 19
Dependent Claim
36 words
A method of manufacturing a bone filling material as claimed in claim 18, wherein said NaOH aqueous solution is heated during said deproteinization at a low temperature between 80°C and 85°C for two to three hours.
References Cited (Backward Citations)
JPH0624994A
Cited
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