Patent EP0742287A2 successfully refreshed from Google Patents.
EP0742287A2
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EP
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Modified nucleic acid probes
Assignee: Affymetrix Inc
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Filing: 1996-05-09
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Granted: —
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Expires: 2016-05-09
Abstract
Abstract Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays.
Inventors
- 👤 Glenn H. Mcgall
- 👤 Charles G. Miyada
- 👤 Maureen T. Cronin
- 👤 Jennifer D. Tan
- 👤 Mark S. Chee
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 18:46
CPC Classifications
C; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
13 words
A composition comprising an array of oligonucleotide analogues attached to a solid substrate.
Claim 2
Dependent Claim
120 words
A composition of claim 1, wherein either:- (a) said array of oligonucleotide analogues comprises a nucleoside analogue with the formula wherein: R 1 is selected from hydrogen, methyl, hydroxy, alkoxy, alkylthio, halogen, cyano, and azido; R 2 is selected from hydrogen, methyl, hydroxy, alkoxy, alkylthio, halogen, cyano, and azido; and Y is a heterocylic moiety; or (b) said array of oligonucleotide analogues comprises a nucleoside analogue with the formula wherein: R 1 is selected from hydrogen, hydroxyl, methyl, methoxy, ethoxy, propoxy, allyloxy, propargyloxy, Fluorine, Chlorine, and Bromine; R 2 is selected from hydrogen, hydroxyl, methyl, methoxy, ethoxy, propoxy, allyloxy, propargyloxy, Fluorine, Chlorine, and Bromine; and Y is a base selected from purines, purine analogues, pyrimidines, pyrimidine analogues, 3-nitropyrrole and 5-nitroindole.
Claim 3
Dependent Claim
25 words
A composition of claim 1, wherein said array of oligonucleotide analogues comprises a nucleotide with a base selected from 7-deazaguanosine, 2-aminopurine, 8-aza-7-deazaguanosine, 1H-purine, and hypoxanthine.
Claim 4
Dependent Claim
52 words
A composition comprising an oligonucleotide analogue array synthesised on a solid substrate, wherein said synthesis is performed by light-directed chemical coupling or by flowing oligonucleotide analogue reagents over predetermined regions of the solid substrate; optionally wherein said solid substrate is derivitised with a silane reagent prior to synthesis of said oligonucleotide analogue.
Claim 5
Independent Claim
39 words
A method of improving the hybridisation of a nucleic acid to an oligonucleotide array, comprising incorporating a base selected from 7-deazaguanosine, 2-aminopurine, 8-aza-7-deazaguanosine, 1H-purine, and hypoxanthine into the oligonucloetides of the array; optionally wherein the oligonucleotide is a homopolymer.
Claim 6
Independent Claim
68 words
A method of determining if a target molecule is complementary to a probe, comprising the steps of: (a) synthesising an oligonucleotide analogue array on a solid substrate; (b) exposing said oligonucleotide analogue array on said solid substrate to a target nucleic acid, optionally after amplification of said target nucleic acid; and (c) determining whether an oligonucloetide analogue member of said oligonucleotide analogue array binds to said target oligonucleotide.
Claim 7
Dependent Claim
21 words
A method of claim 6 wherein said target nucleic acid is selected from genomic DNA, cDNA, unspliced RNA, mRNA, and rRNA.
Claim 8
Dependent Claim
32 words
A composition comprising an array of oligonucleotide probes hybridised to a target nucleic acid, which target nucleic acid comprises a nucleotide analogue; optionally wherein the target nucleic acid is a PCR amplicon.
Claim 9
Independent Claim
39 words
A method of detecting a target nucleic acid, comprising enzymatically copying the target nucleic acid using nucleotides which comprise a nucleotide analogue, thereby producing a nucleic acid analogue amplicon, and hybridising the nucleic acid amplicon to an oligonucleotide array.
Claim 10
Dependent Claim
23 words
A method of claim 9, wherein the oligonucleotide array comprises an oligonucleotide analogue probe which is complementary to the nucleic acid analogue amplicon.
References Cited (Backward Citations)
No citation records indexed.
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