EP0739631A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Laminar bone support for cartilage growth
Assignee: Gendler Family LP
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Filing: 1996-04-25
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Granted: —
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Expires: 2016-04-25
Abstract
Abstract The invention provides a method for producing a biological material comprising reconstituted cartilage tissue, wherein the method comprises isolating chondrocytes from cartilage tissue, contacting a substrate comprised of a continuous, flexible sheet of demineralized natural bone, having a thickness less than about 1.5m, with said chondrocytes, and culturing the chondrocytes on the substrate in growth media to produce a biological material characterized by a continuous layer of cartilage tissue. The invention also provides a biological material made by this method.
Inventors
- 👤 El Gendler
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 21:15
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3641; A61L27/3645; A61L27/3654; A61F; A61F2/00; A61F2/02; A61F2/30; A61F2/30756; A61L27/3604; A61L27/3608; A61L27/3612; A61L27/3683; A61L27/38; C; C12; C12N; C12N5/00; C12N5/0068; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0655; A61F2/28; A61F2/2846; A61F2310/00; A61F2310/00005; A61F2310/00365; A61K; A61K35/00; A61K35/12; A61K38/00; C12N2500/00; C12N2500/30; C12N2500/38; C12N2503/00; C12N2503/02; C12N2533/00; C12N2533/90; Y; Y10; Y10S; Y10S623/00; Y10S623/901
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
35 words
A biological material comprising cartilage tissue reconstituted on a substrate in vitro from isolated chondrocytes wherein the substrate is a continuous, flexible sheet of demineralized natural bone, having a thickness less than about 1.5 mm.
Claim 2
Independent Claim
67 words
A method for producing a biological material comprising reconstituted cartilage tissue, wherein the method comprises isolating chondrocytes from cartilage tissue, contacting a substrate comprised of a continuous, flexible sheet of demineralized natural bone, having a thickness less than about 1.5 mm, with said chondrocytes, and culturing the chondrocytes on the substrate in growth media to produce a biological material characterized by a continuous layer of cartilage tissue.
Claim 3
Dependent Claim
15 words
The method of claim 2 wherein the chondrocytes are isolated from human articular cartilage tissue.
Claim 4
Dependent Claim
15 words
The method of claim 3, wherein the chondrocytes are isolated by sequential enzyme digestion techniques.
Claim 5
Independent Claim
58 words
A method for making a cartilaginous structure comprising providing a substrate comprised of a continuous, flexible sheet of demineralized natural bone, having a 5 thickness less than about 1.5 mm, in a nutrient environment and attaching cartilage cells to the substrate to form a cartilaginous structure suitable for implantation into a patient to replace defective or missing cartilage.
Claim 6
Dependent Claim
31 words
The method of claim 5 further comprising proliferating the cells on the substrate in vitro in a nutrient media, thereby to produce a structure comprising the expanded cells on the substrate.
Claim 7
Dependent Claim
16 words
A biological material as obtainable by the method of any one of claims 2 to 4.
Claim 8
Dependent Claim
14 words
A cartilaginous structure as obtainable by the method of claim 5 or claim 6.
Claim 9
Dependent Claim
24 words
A biological material of claim 1 or claim 7, or a cartilaginous structure of claim 8 for implantation in a human or animal body.
Claim 10
Dependent Claim
33 words
Use of a biological material of claim 1 or claim 7, or of a cartilaginous structure of claim 8 in the manufacture of an implant for implantation in a human or animal body.
Claim 11
Dependent Claim
25 words
Use according to claim 10 wherein the implant is for implantation without prior in vitro proliferation, on the substrate, of cells attached to the substrate.
Claim 12
Dependent Claim
30 words
Use according to claim 10 wherein the implant is for the repair of cartilage damaged by inflammation, trauma or ageing, or for the repair of cartilage which is congenitally defective.
References Cited (Backward Citations)
No citation records indexed.
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