Microplate assembly and method of preparing samples for analysis in a microplate assembly

Abstract

Abstract A microplate assembly (10) for use in analyzing samples captured on a filter medium (16) comprises a carrier plate (12), a holding tray (14), a collimator (18), and a cover film (20). These elements are generally rectangular in shape and are sized to stack on top of one another. The holding tray (14) is positioned within the carrier plate (12), the collimator (18) and filter medium (16) are positioned within the holding tray (14) with the filter medium (16) positioned beneath the collimator (18), and the cover film (20) is sealed over the collimator (18). To prepare samples in the microplate assembly (10) for analysis, the samples are captured on the filter medium (16) and the filter medium (16) is placed in the holding tray (14). After adding scintillation cocktail or luminescent substrate to the filter medium (16), the collimator (18) is placed over the holding tray (14) with the filter medium (16) positioned between the collimator (18) and the holding tray (14) and the samples disposed in the sample wells. The carrier plate (12), the holding tray (14), the filter medium (16), and the collimator (18) are provided with complementary keyed corners (22,34,19,46) to facilitate alignment of these elements relative to one another. The wells of the collimator (18) include respective lower rims (54) protruding into the filter medium (16) to minimize crosstalk through the filter medium (16). The cover film (20) seals the microplate assembly (10) so that the samples are prepared for analysis.

Inventors

• 👤 Alfred J. Kolb
• 👤 Roy L. Manns
• 👤 Kenneth E. Neumann

Bibliographic Details