Patent EP0543612A2 successfully refreshed from Google Patents.
EP0543612A2
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EP
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Nucleic acid target generation
Assignee: Becton Dickinson and Co
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Filing: 1992-11-17
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Granted: —
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Expires: 2012-11-17
Abstract
Abstract The invention is a method for generating nucleic acid sequences ends which comprises; (a) hybridizing a primer to a nucleic acid sequence, (b) hybridizing a primer to the nucleic acid sequence in (a) located 5' to the primer in (a), and (c) polymerizing both primers so that the primer in (a) is displaced from the nucleic acid sequence. The invention provides a method for generating target nucleic acid sequences for subsequent amplification. The method is applicable to both DNA and RNA.
Inventors
- 👤 George Terrance Walker
- 👤 Michel C. Little
- 👤 James G. Nadeau
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 19:33
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/6846; C12Q1/6853
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
54 words
A method for generating nucleic acid sequences which comprises; (a) hybridizing a primer to a nucleic acid sequence, (b) hybridizing a primer to the nucleic acid sequence in (a) located 5' to the primer in (a), and (c) polymerizing both primers so that the primer in (a) is displaced from the nucleic acid sequence.
Claim 2
Independent Claim
54 words
A method for generating nucleic acid sequences which comprises; (a) hybridizing a primer to a nucleic acid sequence, (b) hybridizing a primer to the nucleic acid sequence in (a) located 5' to the primer in (a), and (c) polymerizing both primers so that the primer in (a) is displaced from the nucleic acid sequence.
Claim 3
Dependent Claim
27 words
The method of claim 1 in which two primers are used in each (a) and (b) to produce four doubled stranded copies of the nucleic acid sequence.
Claim 4
Dependent Claim
21 words
The method of claim 1 in which the primer in (a) contains a recognition sequence 5' to the target recognition sequence.
Claim 5
Dependent Claim
38 words
The method of claim 3 in which the recognition sequence located 5' to the target recognition sequence is recognized by Q - beta replicases, restriction enzymes, RNA polymerases, or enzymes that recog - nize origins of replication sequences.
Claim 6
Dependent Claim
39 words
The method of claim 4 in which the recognition sequence is recognized by a restriction enzyme and polymerization takes place in the presence of at least one deoxynucleosidetriphosphate that results in preferential clevage of one strand over the other.
Claim 7
Dependent Claim
15 words
The method of claim 5 in which the recognition sequence recognizes the restriction enzyme Hincll.
Claim 8
Dependent Claim
15 words
The method of claim 5 in which the recognition sequence recognizes the restriction enzyme Ncil.
Claim 9
Independent Claim
60 words
A method for generating nucleic acid sequences which comprises; (a) hybridizing two single stranded primers containing GTTGAC sequences at their 5' ends to two single stranded nucleic acid sequences, (b) hybridizing two single stranded primers to the nucleic acid sequences in (a) located 5' to the primers in (a), and (c) polymerizing all four primers in the presence of dATP(
Claim 10
Independent Claim
60 words
A method for generating nucleic acid sequences which comprises; (a) hybridizing two single stranded primers containing GTTGAC sequences at their 5' ends to two single stranded nucleic acid sequences, (b) hybridizing two single stranded primers to the nucleic acid sequences in (a) located 5' to the primers in (a), and (c) polymerizing all four primers in the presence of dATP(
Claim 11
Dependent Claim
12 words
The method of claim 8 further comprising amplification by strand displacement amplification.
Claim 12
Dependent Claim
8 words
The method of claim 1 further comprising amplification.
References Cited (Backward Citations)
No citation records indexed.
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