EP0531733A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Carrier for animal cell culture
Assignee: Biomaterial Co Ltd
•
Filing: 1992-08-12
•
Granted: —
•
Expires: 2012-08-12
Abstract
Abstract A carrier for animal cell culture, comprising a base material having a three-dimensional network structure, wherein a cell adhesive factor and a cell growth factor are immobilized on a surface of the carrier. The carrier for animal cell culture according to the present invention enables the function and growth of cells to be positively promoted after animal cells are adhered to the carrier.
Inventors
- 👤 Masano Ohno
- 👤 Kimiaki Yasuda
- 👤 Masatoshi Matsumura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 13:03
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/30; C12N2533/70; C12N2533/78
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
33 words
A carrier for animal cell culture, comprising a base material having a three-dimensional network structure, wherein a cell adhesive factor and a cell growth factor are immobilized on a surface of said carrier.
Claim 2
Dependent Claim
23 words
A carrier for animal cell culture according to claim 1, wherein said cell adhesive factor is fibronectin, vitronectin, fibrinogen, gelatin, laminin or collagen.
Claim 3
Dependent Claim
25 words
A carrier for animal cell culture according to claim 1, wherein said cell adhesive factor is a peptide containing an amino acid sequence of Arg-Gly-Asp.
Claim 4
Dependent Claim
25 words
A carrier for animal cell culture according to claim 1, wherein said cell growth factor is hexamethasone, insulin, glucagon, EGF, PDGF, IGF, FGF-family or heparin.
Claim 5
Dependent Claim
25 words
A carrier for animal cell culture according to claim 1, wherein said cell growth factor is a peptide containing an amino acid sequence of Arg-Gly-Phe-Phe.
Claim 6
Dependent Claim
34 words
A carrier for animal cell culture according to any one of claims 1 to 5, wherein said cell adhesive factor and said cell growth factor are linked to said base material through a spacer.
Claim 7
Dependent Claim
16 words
A carrier for animal cell culture according to claim 6, wherein said spacer is a polymer.
Claim 8
Dependent Claim
24 words
A carrier for animal cell culture according to claim 7, wherein said spacer is a polyethyleneimine having a molecular weight of 3,000 or more.
Claim 9
Dependent Claim
34 words
A carrier for animal cell culture according to claim 1, wherein said base material comprises a foam having a porosity of 80% or more and a mean pore diameter of 0.05 to 2.0 mm.
Claim 10
Dependent Claim
52 words
A carrier for animal cell culture according to claim 9, wherein said base material comprises a naturally occurring polymer having a particle diameter of 1.0 to 5.0 mm, a porosity of 97% or more, a mean pore diameter of 0.3 to 2.0 mm and a specific gravity of 1.4 to 1.7 g/cm³.
References Cited (Backward Citations)
US4332916A
Cited
EP0171887A2
Cited
WO1989005616A1
Cited
WO1991001760A1
Cited
EP0416586A1
Cited
EP0420171A1
Cited
WO1991005036A2
Cited
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.