Patent EP0526652A1 successfully refreshed from Google Patents.
EP0526652A1
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Method of type-specific detection of herpes simplex virus
Assignee: Mitsubishi Kagaku Iatron Inc
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Filing: 1992-02-24
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Granted: —
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Expires: 2012-02-24
Abstract
Abstract A method of the type-specific detection of herpes simplex virus, which comprises the step of DNA amplification of a liquid mixture containing either a combination of a first DNA primer containing an at least 15b oligonucleotide part having a base sequence represented by formula (1 a): CACGGGTATA AGGACATCCA with a second DNA primer containing an at least 15b oligonucleotide part having a base sequence represented by formula (2a): GGGTCCTCGT CCAGATCGCT or another combination of a first DNA primer containing an at least 15b oligonucleotide part having a base sequence represented by formula (1 b): GCCTCTTTTC CCCCGGGGAG with a second DNA primer containing an at least 15b oligonucleotide part having a base sequence represented by formula (2b): GGGAAAAAAG CCGCGCCGGGG and a DNA polymerase and an aqueous specimen, and the step of DNA inspection of the resultant reaction solution. The method makes it possible to differentiate infections diseases caused by HSV type I and HSV type II accurately, rapidly and type-specifically.
Inventors
- 👤 Toshiya Matsumoto
- 👤 Takashi Kurimura
- 👤 Hiroshi Kita
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 13:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/705
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
136 words
A method of the type-specific detection of herpes simplex virus, characterized by comprising the step of DNA amplification in a liquid mixture containing either of a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1 a) with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2a): or a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1 b): with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2b): a DNA polymerase and an aqueous liquid sample; and then the step of DNA examination on the resultant reaction solution.
Claim 2
Independent Claim
136 words
A method of the type-specific detection of herpes simplex virus, characterized by comprising the step of DNA amplification in a liquid mixture containing either of a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1 a) with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2a): or a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1 b): with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2b): a DNA polymerase and an aqueous liquid sample; and then the step of DNA examination on the resultant reaction solution.
Claim 3
Dependent Claim
64 words
A method according to claim 1, wherein said combination of said first DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (1 a) and said second DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (2a) is used to detection specific to the herpes simplex virus Type I.
Claim 4
Dependent Claim
36 words
A method according to claim 1 or 2, wherein said first DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (1 a) is 20 mer to 25 mer.
Claim 5
Dependent Claim
36 words
A method according to claim 1 or claim 2, wherein said second DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (2a) is 20 mer to 25 mer.
Claim 6
Dependent Claim
64 words
A method according to claim 1, wherein said combination of said first DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (1 b) and said second DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (2b) is used to detection specific to the herpes simplex virus Type II.
Claim 7
Dependent Claim
36 words
A method according to claim 1 or 5, wherein said first DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (1 b) is 20 mer to 25 mer.
Claim 8
Dependent Claim
35 words
A method according to claim 1 or 5, wherein said second DNA primer containing the oligonucleotide having at least 15 bases in the base sequence of the formula (2b) is 20 mer to 25 mer.
Claim 9
Independent Claim
90 words
A method according to any one of claims 1 to 7, wherein said DNA amplification comprises the steps of: (i) denaturing a double strand DNA (for about 10 seconds to 2 minutes at about 90 °C to 95 C), (ii) annealing a single strand DNA with the first and second primers (for about 30 seconds to about 3 minutes at about 37 °C to 70 C), and (iii) extending a DNA by a DNA polymerase (for about 30 seconds to about 5 minutes at about 65°C to 80 ° C).
Claim 10
Independent Claim
49 words
A method according to any one of claims 1 to 7, wherein said DNA detection step is a gel electrophoresis, an ethidium bromide dyeing method, a Southern blotting hybridization, an in situ hybridization method, a determination of a base sequence by a dideoxy method, or a radioactive labeling method.
Claim 11
Independent Claim
50 words
A method of the type-specific detection of herpes simplex virus, characterized by bringing an oligonucleotide probe having at least 10 bases in a base sequence of the formula (3): or the formula (4) and carrying a label thereon into contact with a sample; and detecting a signal from the label.
Claim 12
Independent Claim
50 words
A method of the type-specific detection of herpes simplex virus, characterized by bringing an oligonucleotide probe having at least 10 bases in a base sequence of the formula (3): or the formula (4) and carrying a label thereon into contact with a sample; and detecting a signal from the label.
Claim 13
Dependent Claim
37 words
A method according to claim 10, wherein said probe containing the oligonucleotide having at least 10 bases in the base sequence of the formula (3) is used to detection specific to the herpes simplex virus Type I.
Claim 14
Dependent Claim
37 words
A method according to claim 10, wherein said probe containing the oligonucleotide having at least 10 bases in the base sequence of the formula (4) is used to detection specific to the herpes simplex virus Type II.
Claim 15
Dependent Claim
34 words
A method according to claim 1, wherein said probe containing the oligonucleotide having at least 10 bases in the base sequence of the formula (3) or (4) is used in said DNA examination step.
Claim 16
Dependent Claim
32 words
A method according to claim 2, wherein said probe containing the oligonucleotide having at least 10 bases in the base sequence of the formula (3) is used in said DNA examination step.
Claim 17
Dependent Claim
32 words
A method according to claim 5, wherein said probe containing the oligonucleotide having at least 10 bases in the base sequence of the formula (4) is used in said DNA examination step.
References Cited (Backward Citations)
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