Patent EP0476192A2 successfully refreshed from Google Patents.
EP0476192A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Characterizing matter of biological origin using near infrared spectrocopy
Assignee: University of Washington
•
Filing: 1990-09-18
•
Granted: —
•
Expires: 2010-09-18
Abstract
Abstract A method is provided for predicting a property of a matter of biological origin, such as biological fluid, containing water, where the biological matter may be approximated to contain two compartments where one compartment has a proportionally larger or smaller amount of water than the other compartment having the property of interest. The method involves establishing a training set in the near-infrared (NIR) region with independent quantification of the property of the matter using known techniques. The training set is mathematically analyzed according to a correlation developed by regression analysis after employment of a pre-processing technique. The result is a mathematical transformation equation which quantitatively relates spectral intensities at specific wavelengths to the property of interest. This transformation equation may be applied to unknown samples so as to predict their properties, thereby eliminating need for the reference method, except for validation and recalibration. The method provides rapid and accurate prediction of the property of the unknown sample, which may be the property of hematocrit or hemoglobin concentration in whole animal blood. The pre-processing technique may be the transformation of the spectra using a multiple derivative calculation, such as computing the second derivative of the several spectra of known and unknown samples.
Inventors
- 👤 James B. Board Of Regents Univ.Washington Callis
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 12:59
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/31; G01N21/35; G01N21/359; G01N2021/3196
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
140 words
A method for analyzing a property of biological matter having a water content, the biological matter comprising a first compartment related to the property to be analyzed and a second compartment having a proportionally larger or smaller amount of water than the first compartment, the method comprising: (a) irradiating with near infrared light multiple samples of biological matter; (b) detecting the near infrared spectrum of each of said multiple samples; (c) applying a pre-processing technique to the spectrum of each of said multiple samples; (d) independently quantifying the property to be analyzed for each of said multiple samples; (e) establishing a training set from said near infrared spectra of said multiple samples; and (f) statistically identifying the nature of a mathematical correlation between the property to be analyzed in the first compartment and the water content in the biological matter.
Claim 2
Dependent Claim
70 words
A method according to Claim 1, further comprising the steps of: (g) irradiating an unknown sample of the biological matter with near infrared light; (h) detecting the near infrared spectrum of said unknown sample; (i) applying said pre-processing technique to said spectrum of said unknown sample; and (j) predicting the property to be analyzed in said unknown sample by utilizing said mathematical correlation obtained in said statistically identifying step (f).
Claim 3
Dependent Claim
29 words
A method according to Claim 1, wherein said statistically identifying step (f) uses linear regression analysis, multiple linear regression analysis, stepwise regression analysis, or partial least squares regression analysis.
Claim 4
Dependent Claim
45 words
A method according to Claim 1 or Claim 2, wherein said mathematical correlation in said statistically identifying step (f) comprises a linear function related to a near infrared absorbance peak of water in the absorbance spectra of said multiple samples subjected to said pre-processing technique.
Claim 5
Dependent Claim
32 words
A method according to Claim 4, wherein said pre-processing technique comprises transforming said spectra of said multiple samples of said training set spectra by computing a multiple derivative of said multiple samples.
Claim 6
Dependent Claim
14 words
A method according to Claim 5, wherein said multiple derivative is the second derivative.
Claim 7
Dependent Claim
37 words
A method according to Claim 4, wherein said near infrared absorbance peak of water is attributable to combinations of the symmetric O-H stretching, the antisymmetric O-H stretching and the O-H bending mode in a molecule of water.
Claim 8
Dependent Claim
35 words
A method according to Claim 1 or Claim 2, wherein the biological matter is whole blood and the property of the first compartment to be analyzed is hematocrit or hemoglobin concentration in the whole blood.
Claim 9
Dependent Claim
32 words
A method according to Claim 4, wherein the biological matter is whole blood and said absorbance peak of water occurs in the near infrared spectra from about 1150 to about 1190 nanometers.
Claim 10
Dependent Claim
32 words
A method according to Claim 5, wherein the biological matter is whole blood and said absorbance peak of water occurs in the near infrared spectra from about 1150 to about 1190 nanometers.
Claim 11
Dependent Claim
37 words
A method according to Claim 2, wherein said detecting step (b) and said detecting step (h) use spectral analysis instrumentation which records said absorbance spectra of said multiple samples and said unknown sample in a static condition.
Claim 12
Dependent Claim
67 words
A method according to Claim 2, wherein the property to be analyzed is hematocrit and said mathematical correlation solves the equation: Y = b + m * (Second Derivative of the spectral intensity at wavelength X) where Y is the Hematocrit; b ranges from about 18 to about 23; m ranges from about 419 to about 454; and X ranges from about 1160 to about 1165 nm.
Claim 13
Dependent Claim
71 words
A method according to Claim 2, wherein the property to be analyzed is concentration of hemoglobin and said mathematical correlation solves the equation: Y = b + m * (Second Derivative of the spectral intensity of wavelength at X) where Y is the Concentration of Hemoglobin; b ranges from about 6.45 to about 7.6; m ranges from about 145 to about 159; and X ranges from about 1160 to about 1165.
Claim 14
Dependent Claim
29 words
A method according to Claim 1, wherein said mathematical correlation statistically identified in step (f) indicates a complementary relationship between the property to be analyzed and the water content.
Claim 15
Dependent Claim
25 words
A method according to Claim 1 or Claim 2, wherein said multiple samples are of at least one known organism of a given biological species.
Claim 16
Dependent Claim
84 words
A method according to Claim 15, further comprising the steps of: (1) obtaining additional samples of biological matter from an organism of said given species; (2) performing steps (a), (b), (c), and (d) with respect to said additional samples; (3) predicting the property to be analyzed in said additional samples by utilizing said mathematical correlation obtained in said statistically identifying step (f); and (4) validating said mathematical correlation by comparing the property predicted in step (3) to the property independently quantified in step (d).
Claim 17
Independent Claim
117 words
A method for the analysis of a biological fluid having a water content, comprising: gathering near-infrared spectral data of multiple samples of a biological fluid of at least one organism, where the biological fluid may be approximated to comprise two compartments where one compartment has a proportionally different amount of water than the other compartment which has a property of interest; determining the best absorbance detection point comprising an absorbance peak of the water content in the near infrared spectrum of each of said multiple samples; independently measuring the property to be analyzed in each of said multiple samples; identifying a mathematical correlation of the property to be analyzed and the water content in the biological fluid.
Claim 18
Dependent Claim
41 words
A method according to Claim 17, further comprising: analyzing an unknown sample of the biological fluid to predict the property to be analyzed in said unknown sample by applying said mathematical correlation to a near infrared spectrum of said unknown sample.
Claim 19
Dependent Claim
62 words
A method according to Claim 18, wherein said determining further comprises transforming the absorbance peak of the water content of each spectrum of each of the samples by computing a multiple derivative of each spectrum and wherein said analyzing further comprises transforming the near infrared spectrum of said unknown sample by computing a multiple derivative of the spectrum of the unknown sample.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.