Patent EP0474979A1 successfully refreshed from Google Patents.
EP0474979A1
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Heparin binding neurotrophic factor gene sequence
Assignee: Wyeth Holdings LLC
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Filing: 1991-06-11
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Granted: —
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Expires: 2011-06-11
Abstract
Abstract The invention relates to Novel DNA and amino acid sequences for a heparin bending neurotrophic factor, (HBNF). Also described are expression vectors and host cells useful in a method for production of the HBNF protein.
Inventors
- 👤 Imre Kovesdi
- 👤 Peter Bohlen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 03:22
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C07; C07K; C07K14/00; C07K14/435; C07K14/475; A; A61; A61P; A61P25/00; A61K; A61K38/00; A61K48/00
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
12 words
A purified and isolated gene encoding a heparin binding neurotrophic factor (HBNF).
Claim 2
Dependent Claim
12 words
A purified and isolated gene encoding a heparin binding neurotrophic factor (HBNF).
Claim 3
Dependent Claim
24 words
The gene of Claim 1 which has the sequence depicted in Figure 1, or a portion thereof, which encodes a biologically active HBNF protein.
Claim 4
Dependent Claim
20 words
The gene of Claim 1 which is hybridizable with the sequence depicted in Figure 1 under standard high stringency conditions.
Claim 5
Dependent Claim
38 words
A method for production of substantially pure HBNF protein which comprises transforming a host cell with the gene of Claim 1 and culturing the host cell under conditions which permit expression of the gene by the host cell.
Claim 6
Dependent Claim
9 words
An expression vector comprising the gene of Claim 1.
Claim 7
Dependent Claim
9 words
A host cell comprising the gene of Claim 1.
Claim 8
Dependent Claim
17 words
The cell of Claim 6 which is deposited with the American Type Culture Collection as ATCC 68385.
Claim 9
Dependent Claim
25 words
A purified isolated HBNF protein having a sequence depicted in Figure 1 or Figure 5. and homologues or fragments thereof which retain HBNF biological activity.
Claim 10
Dependent Claim
25 words
A purified isolated HBNF protein having a sequence depicted in Figure 1 or Figure 5. and homologues or fragments thereof which retain HBNF biological activity.
Claim 11
Dependent Claim
20 words
A therapeutic composition comprising an effective amount of the protein of Claim 8, in combination with a pharmaceutically acceptable carrier.
Claim 12
Dependent Claim
30 words
A method of maintaining or promoting growth of nerve cells in vitro which comprises culturing the cells in the presence of an effective amount of the protein of Claim 8.
Claim 13
Dependent Claim
37 words
A method of repairing or treating damaged nerve cells in vivo which comprises administering to an individual in need of such treatment an effective amount of compatible transgenic cells capable of expressing the protein of Claim 8.
Claim 14
Independent Claim
21 words
A method for inducing differentiation of undifferentiated cells which comprises applying to the cells an effective amount of an HBNF protein.
Claim 15
Independent Claim
21 words
A method for inducing differentiation of undifferentiated cells which comprises applying to the cells an effective amount of an HBNF protein.
References Cited (Backward Citations)
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