EP0465572B1
Expired / Lapsed
EP
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Genetic engineering of novel plant phenotypes
Assignee: DNA Plant Technology Corp
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Filing: 1990-03-30
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Granted: 1995-06-14
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Expires: 2010-03-30
Abstract
Abstract The invention provides a plant in which expression of an endogenous gene has been reduced and wherein the genome of cells thereof is modified by incorporation of an additional DNA segment, transcripts of which segment are produced in the sense orientation and thus produced transcripts having a sequence complementary to said endogenous gene.
Inventors
- 👤 Richard A. Jorgensen
- 👤 Carolyn A. Napoli
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 13:55
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8201; C12N15/8213; C12N15/8216; C12N15/8218; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/825; C12N9/10; C12N9/1025; C12N9/1029; C12N9/1037; C12N9/88; C12Y; C12Y101/00; C12Y101/01; C12Y101/01219
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
99 words
A method for reducing production of a protein product of an endogenous gene in a plant, said protein affecting a phenotypic trait in the plant, said method comprising the steps of: growing plants from a plant cell transformed with a polynucleotide comprising a DNA segment under operational control of a promoter, whereby said segment is transcribed in the cell in the sense orientation and wherein said DNA segment has a sequence complementary to mRNA encoded by said endogenous gene; and identifying a plant that exhibits a phenotypic change by screening the resulting plants for modification of the phenotypic trait.
Claim 2
Dependent Claim
12 words
A method of claim 1, wherein said plant is a flowering plant.
Claim 3
Dependent Claim
82 words
A method of claim 1 wherein said plant is selected from the genera Fragaria, Lotus, Medicago, Onobrychis, Trifolium, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Arabidopsis, Brassica, Raphanus, Sinapis, Atropa, Capsicum, Hyoscyamus, Lycopersicon, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Ciohorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Hererocallis, Nemesia, Pelargonium, Panicum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Browaalia, Glycine, Lolium Zea, Triticum, Sorghum, Datura, Chrysanthemum, Dianthus, Gerbera, Euphorbia, Pelaronium, Ipomoea, Passiflora, Cyclamen, Malus, Prunus, Rosa, Rubus, Populus, Santalum, Allium, Lilium, Narcissus, Ananas, Arachis, Phaseolus and Pisum.
Claim 4
Independent Claim
17 words
A method of any one of claims 1 to 3, wherein said segment encodes a full-length protein.
Claim 5
Independent Claim
17 words
A method of any one of claims 1 to 4, wherein said promoter is a constitutive promoter.
Claim 6
Independent Claim
18 words
A method of any one of claims 1 to 4, wherein said promoter is exogenous to said plant.
Claim 7
Independent Claim
30 words
A method of any one of claims 1 to 6, wherein said segment transcripts have at least 80%, optionally 95% to 100% sequence identity to transcripts of said endogenous gene.
Claim 8
Independent Claim
18 words
A method of any one of claims 1 to 7, wherein said polynucleotide is in a DNA vector.
Claim 9
Independent Claim
19 words
A method of any one of claims 1 to 7, wherein said polynucleotide is in a Ti plasmid vector.
Claim 10
Independent Claim
34 words
A method of any one of claims 1 to 9, wherein a transcript of said segment in the transgenote cells is identical to a transcript of the endogenous gene sequence in the transgenote cells.
Claim 11
Independent Claim
21 words
A method of any one of claims 1 to 10, wherein said segment is at least about 50 nucleotides in length.
Claim 12
Independent Claim
114 words
A method for producing a plant exhibiting at least one modified phenotypic trait resulting from reduced expression of an endogenous gene in said plant, comprising: transforming plant cells with a polynucleotide to produce transgenote cells, the polynucleotide comprising a promoter operably linked to a DNA segment such that transcripts of the segment are produced in the sense orientation in the transgenote cells, which segment transcripts have at least 65% sequence identity to transcripts of said endogenous gene; growing plants from one or more of said transgenote cells, wherein production of mRNA encoded by the endogenous gene is reduced in one or more of the plants; and selecting a plant exhibiting said modified phenotypic trait.
Claim 13
Dependent Claim
19 words
A method of claim 12 further defined by the specific feature(s) of any one of claims 2 to 11.
Claim 14
Dependent Claim
46 words
The use of a vector in transforming plant cells and reducing expression in said cells of an endogenous gene, said vector comprising at least a portion of the sequence of said gene operably linked to a promoter and transcribable to produce mRNA in the sense orientation.
Claim 15
Dependent Claim
51 words
The use of a DNA sequence in modifying the genome of a plant to reduce expression of a pre-existing gene of said plant, which sequence is capable of transcription in a sense orientation with respect to said pre-existing gene and comprises a sequence complementary to mRNA encoded by said pre-existing gene.
Claim 16
Dependent Claim
56 words
The use of a DNA sequence in modifying the genome of a plant to reduce expression of a pre-existing gene of said plant, which sequence is capable of transcription in a sense orientation with respect to said pre-existing gene and transcripts of said sequence having at least 65% sequence identity to transcripts of said pre-existing gene.
References Cited (Backward Citations)
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